The advancement of highly boosted internal combustion engines (ICEs) with high thermal efficiency is mainly constrained by knock and super-knock, respectively, due to the end gas autoignition detonation development. pressure wave propagation reflection in a small confined space may strongly interact local autoignition, leading characteristics different from those large chamber or open space. pr...

Leishmania parasites include important pathogens and model organisms are even used for the production of recombinant proteins. However, functional genomics characterization essential genes often limited in because low-throughput technologies gene disruption or tagging absence components RNA interference. Here, we tested T7 polymerase-dependent CRISPR-Cas9 system by Beneke et al. glmS ribozyme-b...