results fifty isolates were positive on the screening test. among them, positive pcr reactions were identified in 35/221 and 12/77 isolates of e. coli and klebsiella spp., respectively, including 16 (34.0%) for cit only, 7 (14.8%) for dha only, and 24 (51.0%) for both dha and cit. no isolate was positive for fox or mox. no proteus organism was positive for ampc genes. conclusions currently, phe...

Sir, Carbapenems are often the last active antibiotics for serious infections caused by Gram-negative opportunist pathogens. Resistance is still extremely rare among Enterobacteriaceae, but is increasingly detected in isolates of Klebsiella and Enterobacter in the UK. In a few cases it is mediated by class A or B carbapenemases, but more often results from combinations of a b-lactamase [often a...

BACKGROUND Production of β-lactamase enzymes is the most common and important mechanism of resistance in Gram-negative bacteria. The objective of this study was to assess frequency of three main β-lactamase enzymes, including extended spectrum β-lactamases (ESBLs), metallo-β-lactamase (MBL), and Klebsiella pneumoniae carbapenemase (KPC) enzymes in Escherichia coli and Klebsiella spp. isolated f...

The Australian Group on Antimicrobial Resistance (AGAR) performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2010 survey focussed on community-onset infections, examining isolates from urinary tract infections from patients presenting to outpatient clinics, emergency departments or to community practitioners. ...

BACKGROUND & OBJECTIVE Extended spectrum beta-lactamase (ESBL) producing Klebsiella spp led to serious concern about septicaemic neonates in neonatal intensive care units (NICU) due to high resistance against commonly used antimicrobial agents. Knowledge of disease burden and information on resistance to antimicrobials are required for proper management of such cases in NICUs. Here we report th...

The VITEK 2 (bioMérieux Inc., Hazelwood, MO) automated system employs an advanced expert system (AES) analyzing antimicrobial susceptibility test results according to the bacterial species (2). According to the AES, the piperacillin susceptibilities of all Klebsiella spp. are converted to resistance even though the automated MICs determined for their isolates are low. In contrast, other automat...

The aim of the study was to assess microbial quality of water in dental unit waterlines in a dental school at Mangalore. Methodology: Water samples were taken from tap water for control, air/water syringe and the high speed hand piece of 20 dental units. The samples were transferred to the laboratory and streaked on the culture media namely Mac Conkey agar, Sabourauds dextrose media, Buffered c...

Objective: The ESBL producing organisms has been steadily increasing over the past years. The detection and treatment of these ESBL organisms are extremely limited. In the present study, to determine the Extended Spectrum Beta Lactamases producing organism which were isolated from various samples of Multispeciality hospital in Salem, India. Methods: A total of 278 gram negative isolates were re...

In this study, urinary catheter utilization rates, the causative agents for catheter-associated urinary tract infection (CAUTI) and their antimicrobial susceptibilities in intensive care units (ICUs) in 2009 were investigated at Gazi university hospital. We aimed to determine the causative agents and risk factors for CAUTIs, and antimicrobial susceptibilities of the pathogens; and also sensitiv...

In a study of 1,777 Klebsiella strains, we discovered KPN1705, which was distinct from all recognized Klebsiella spp. We closed the genome of strain KPN1705 using a hybrid of Illumina short-read and Oxford Nanopore long-read technologies. For this novel species, we propose the name Klebsiella quasivariicola sp. nov.