To verify this assumption we evaluated another kit, the “/3hCG MAIA-clone immunoradiometric assay” (Serono), comparing the effects of lipemia on the results obtained with it and with Clinical Assays’ monoclonal antibody kits; we used sera from seven male patients, selected for hipemia. We also examined the effects of free fatty acids at concentrations of 1 g/L in hCG-free serum. Both assays wer...

In de novo genome sequencing, mate-pair reads are crucial for scaffolding assembled contigs. However, preparation of mate-pair libraries is not a trivial task, even when using one of the latest approaches, the Nextera Mate Pair Sample Prep Kit from Illumina. To reduce cost and enhance library yield and fidelity when using this kit, we have modified the manufacturer's protocol based on (i) varia...

Lung scanning has been recognized as a clinically useful technique for portraying macroscopic pulmonary perfusion defects since it was first reported by Wagner in 1964 (1 ). A number of radiopharmaceuticals have been proposed to assess pulmonary perfusion. Although 1 3 1 I imposes many limitations as a radiotagging agent, the use of iodinated macroaggregated albumin (I-MAA) has received wide ac...

The expression and function of a receptor tyrosine kinase, c-kit, in the adult bone marrow of the mouse were investigated by using monoclonal antibodies (mAbs) against the extracellular domain of murine c-kit. In adult C57BL/6 mouse, 7.8% of total bone marrow cells express c-kit on their surface. Half of the c-kit+ cells do not express lineage markers including Mac-1, Gr-1, TER-119, and B220, w...

Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality...

OBJECTIVE Gastrointestinal stromal tumors (GISTs) with no mutations in exons 9, 11, 13, and 17 of the KIT gene and exons 12, and 18 of the PDGFRA gene were defined as KIT/PDGFRA wild-type and they accounted for ~15-20% of GISTs. However, some KIT/PDGFRA wild-type GISTs with KIT mutations in other exons were occasionally reported. We therefore assessed GISTs to understand the whole genomic genot...