( a ) Microsomes were incubated with [ 'Tlserine (2 mM) in 40 mM-Hepes buffer, pH 7.4, containing 0.25 M-sucrose and 1 mM-CaCI,. After each incubation time the microsomes were treated with TNBS and the lipids extracted and analysed (Hutson & Hig ins, 1985). ( h ) Hepatocytes were incubated with 2 mwj"C7serine in Krebs-Henseleit buffer. The cells and medium were separated by centrifugation and t...

Activation of beta-adrenergic receptors with isoproterenol fails to stimulate prostaglandin synthesis in the isolated rabbit heart perfused with Ca2+-free Krebs-Henseleit buffer. This lack of response could be due to reduced isoproterenol-stimulated liberation of arachidonic acid, reduced cyclooxygenase activity during Ca2+-free perfusion, or both. To test these hypotheses, we have examined the...

Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical ( . OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe 2+ -O2 and Cu + -O2 chemistry is capable of generating . OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe 2+ -O2 system and of the Fenton system (Fe 2+ + H2O2) t...

Tetraethylammonium (TEA) and amantadine are two organic cations that are secreted by the kidney. It appears that each cation may characterize distinct renal tubule organic cation transport pathways. To test this hypothesis, we investigated the renal proximal and distal tubule energy-dependent transport properties of TEA and amantadine. Isolated tubules were incubated at 25 degrees C in bicarbon...

Abstract This study presents a detailed analysis of the lateral forces generated as result vertically applied loads to recycled plastic drainage kerbs. These kerbs are relatively new addition road infrastructure projects. When concrete is used form kerbs, its deformation minimum when stressed under heavy axle loads. Although more environmentally friendly construction product, they less stiff th...

t-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the fusarium species. fusarium species have been recognized as a great agricultural problem. they occur worldwide on a variety of plant hosts and cereal grains. the aim of this study was to investigate t-2 toxin-induced liver injury using in situ perfused rat liver....

Methods Heart Perfusion Protocol Male Sprague Dawley rats were anesthetized. The heart was excised, cannulated, and perfused with Krebs-Henseleit buffer equilibrated with 95% O2 5% CO2 at 370C. A water-filled latex balloon was inserted into the left ventricle and connected to a pressure transducer to record the left ventricular pressure and heart rate. The rate-pressure product (RPP) was used a...

BACKGROUND We investigated whether intermittent blood and crystalloid cardioplegia differentially affect myocardial apoptosis and apoptosis gene-related proteins. METHODS AND RESULTS Rabbit hearts were perfused with Krebs-Henseleit buffer on a Langendorff apparatus. Control hearts (n=6) were perfused for 120 minutes without cardioplegic ischemia. Hearts were arrested for 60 minutes with warm ...

AIM To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae). METHODS The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12...

liver injury induced by viruses, chemicals and drugs can be protected by different medicinal plants. Feijoa sellowiana (Myrtaceae) is an evergreen bush native to southern areas of South America, as well as Iran where the fruits are very popular. Feijoa has shown a potent antimicrobial effect. Morever, the antioxidant activity of total Feijoa extract has also been reported. MDMA or ecstasy is a ...