in order to study the effect of ca, k, and mn foliar spray on vegetative traits of safflower (padideh cultivar) under salt stress, a factorial experiment was conducted based on randomized complete blocks design with three replications in vali-asr university greenhouse. factors included salinity with four levels representing 0, 500, 1000, and 1500 mg nacl kg -1, and foliar spray with four level ...

The present study illustrates the optimization and characterization of β-glucosidase from a bacterial isolate, strain SG9. Sixty-eight different variables were first screened by one factor at a time method. The screened variable optimization was then performed by Plackett-Burman design followed by Box-Behnken response surface methodology. Thirty-one variables were screened, of which five variab...

Polar microorganisms produce physiologically active substances to adapt harsh environments, and these can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica, produced phytol, a natural antimicrobial agent. Furthermore, several polyunsaturated fatty acids (PUFAs), including omega-3, exhibit antioxidant properties. Here statisti...

was isolated from a soil sample collected in Jiangsu Province, People's Republic of China. In this communication, we would like to report the production, isolation and structural elucidation of 8-methoxygriseorhodin C. The producing strain was inoculated in a 500-ml baffled Erlenmeyer flask containing 1 10 ml medium consisting of glycerol 2.0%, soybean meal 1.5%, CoCl3-6H2O 0.0005% and K2HPO4 0...

A miniaturized extraction/preconcentration method based on an aqueous biphasic system (μ-ABS) was developed with reagents commonly used as food additives: cholinium chloride (ChCl) main extraction phase, K2HPO4 salting-out agent, and water the component (being sample for analyses). With aim of obtaining high enrichment factors, miniaturization, adequate analytical performance, a point in region...

Media The motility buffer (MB) contained (/l of distilled water) K2HPO4, 11.2 g; KH2PO4, 4.8 g; (NH4)2SO4, 2 g; MgS04. 7H20, 0.25 g; PVP, 1 g; and EDTA, 0.029 g.1 LuriaBertani (LB) broth contained LB (/l of distilled water) 25 g. Tryptone medium contained (/l of distilled water) tryptone, 10 g and Nacl, 5 g. Different concentrations of chemotaxis medium was prepared by adding different amounts ...

The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l), potassium phosphate (175 g l, K2HPO4 +KH2PO4) and glucose (10 gl), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-.