A unique oligonucleotide pair, GOCC56:GOCC427, was designed that correctly primed specific amplification of a approximately 370-bp sequence spanning the ITS and 5.8S rDNA regions of Glomus occultum and Glomus brasilianum. In addition, this primer pair successfully detected G. occultum and G. brasilianum DNA in nested PCR using a primary PCR product amplified from highly diluted extracts of colo...

Terminal restriction fragment length polymorphism (TRFLP) profiling of the internally transcribed spacer (ITS) ribosomal DNA of unknown fungal communities is currently unsupported by a broad-range enzyme-choosing rationale. An in silico study of terminal fragment size distribution was therefore performed following virtual digestion (by use of a set of commercially available 135 type IIP restric...

In May 2019, mature jackfruit (Artocarpus heterophyllus) fruits were found infected with fan-shaped white mycelia and brown to black sclerotia at the Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh. The fungus was successfully isolated on potato dextrose agar medium. internal transcribed spacer region of rDNA amplified using primer pair ITS1 ITS4. Based morphology molecul...

The objective of this present study was to isolate a potential laccase producing fungi from the different environmental samples from Gujarat. Eighteen morphologically different fungi were isolated on mineral salts medium having lignin (sole carbon source) and screened qualitatively for their ability to produce laccase by plate screening method using the indicators ABTS, Brilliant Blue R (RBBR),...

The purpose of this work was to evaluate the antimicrobial potential of endophytic fungi isolated from different high value medicinal plants of Kashmir valley. Evaluation of some endophytes has been carried out for their possible antimicrobial activity from various parts of medicinal plants belonging to Kashmir valley (India). A total of twenty eight fungal endophytes were isolated from the dif...

Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies b...

Severe epidemics of leaf blotch and black leaf spot of oat (Avena sativa) caused by Drechslera avenae and Drechslera sp., respectively, are frequently observed in the State of Paraná, Brazil. Although some morphological differences between the isolates causing two different symptoms were noticed, the genetic relationship between them was not clear. Twenty-four isolates of D. avenae and Drechsle...

Phytophthora and Pythium are a group of Oomycetes that widely associated with diseases in potato plants. Therefore, this study was conducted to identify the rhizosphere infected plants showing leaf blight. Four isolates were collected from four regions Central Java (UGM_St_TM, UGM_St_BK, UGM_St_BNJ, UGM_St_KJ, isolate Temanggung, Bakal, Banjarnegara, Kejajar respectively), UGM_St_NG as culture ...

Tomato plants displaying early blight symptoms were collected from different localities in the provinces of Assiut and Sohag, Egypt. The causal pathogens isolated on potato dextrose agar plates. Pathogenicity tests with 48 isolates carried out under greenhouse conditions tomato cultivar (CV 844). All tested caused disease degrees. highest severity was found after inoculation isolate No. 6 follo...