نتایج جستجو برای: its2 وrdna وpcr

تعداد نتایج: 1660  

2015
Yue Liu Wei Sun Chuan Liu Yaqin Zhang Yilong Chen Ming Song Gang Fan Xia Liu Li Xiang Yi Zhang

BACKGROUND The morphological identification of different Hippophae species (Shaji) was difficult. This study aims to discriminate between medicinal and non-medicinal Hippophae species by DNA barcodes, the ITS2, psbA-trnH, and a combination of ITS2 and psbA-trnH (ITS2 + psbA-trnH). METHODS DNA was extracted from the dried fruit samples. Primer pairs ITS2F/3R for ITS2 and psbAF/trnHR for psbA-t...

Journal: :Molecular biology and evolution 1994
G N Fritz J Conn A Cockburn J Seawright

Sequence variation of the ribosomal DNA internal transcribed spacer 2 (ITS2) was examined for populations of the malaria vector Anopheles nuneztovari collected in Colombia, Venezuela, Bolivia, Suriname, and Brazil. Mosquitoes from Colombia and Venezuela had identical ITS2 sequences and were distinguished from sequences in other populations by three insertion/deletion events (indels) and by one ...

Hamidreza Haddadzadeh Parviz Shayan حمیدرضا حدادزاده,

Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...

Journal: :RNA 2005
Jörg Schultz Stefanie Maisel Daniel Gerlach Tobias Müller Matthias Wolf

The ongoing characterization of novel species creates the need for a molecular marker which can be used for species- and, simultaneously, for mega-systematics. Recently, the use of the internal transcribed spacer 2 (ITS2) sequence was suggested, as it shows a high divergence in sequence with an assumed conservation in structure. This hypothesis was mainly based on small-scale analyses, comparin...

2010
Hui Yao Jingyuan Song Chang Liu Kun Luo Jianping Han Ying Li Xiaohui Pang Hongxi Xu Yingjie Zhu Peigen Xiao Shilin Chen

BACKGROUND The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is regarded as one of the candidate DNA barcodes because it possesses a number of valuable characteristics, such as the availability of conserved regions for designing universal primers, the ease of its amplification, and sufficient variability to distinguish even closely related species. However, a general anal...

2010
Shilin Chen Hui Yao Jianping Han Chang Liu Jingyuan Song Linchun Shi Yingjie Zhu Xinye Ma Ting Gao Xiaohui Pang Kun Luo Ying Li Xiwen Li Xiaocheng Jia Yulin Lin Christine Leon

BACKGROUND The plant working group of the Consortium for the Barcode of Life recommended the two-locus combination of rbcL+matK as the plant barcode, yet the combination was shown to successfully discriminate among 907 samples from 550 species at the species level with a probability of 72%. The group admits that the two-locus barcode is far from perfect due to the low identification rate, and t...

2017
Qian Li Jinguang Xu Lu Han Chengrong Gao Jie Lu Guangcong Du Xia Sun

Herbaceous peony (Paeonia lactiflora Pall.) is an important ornamental and medicinal plant. DNA barcodes can reveal species identity via the nucleotide diversity of short DNA segments. In this study, two main candidate DNA barcodes (ITS2 and psbA-trnH) were tested to identify twenty-one cutting cultivars of P. lactiflora and their wild species. The efficacy of the candidate DNA barcodes was ass...

Journal: :Nucleic acids research 1999
N Joseph E Krauskopf M I Vera B Michot

Molecular mechanisms of ITS2 processing, a eukaryotic insertion between the 5.8S and LSU rRNA, remain largely elusive even in yeast. To delineate ITS2 structural and functional features which could be common to eukaryotes, we first produced phylo-genetically supported folding models in the vertebrate lineage, then tested them in deeper branchings and, more particularly, among yeasts. ITS2 compa...

2015
Nazar A. Shapoval Vladimir A. Lukhtanov

The eukaryotic ribosomal DNA cluster consists of multiple copies of three genes, 18S, 5. 8S and 28S rRNAs, separated by multiple copies of two internal transcribed spacers, ITS1 and ITS2. It is an important, frequently used marker in both molecular cytogenetic and molecular phylogenetic studies. Despite this, little is known about intragenomic variations within the copies of eukaryotic ribosoma...

2017
Jana Batovska Noel O I Cogan Stacey E Lynch Mark J Blacket

Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (SNPs), insertions/deletions (indels), homopolymeric regions, and micros...

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