Techniques of isothermal microcalorimetry have been much improved during the past two decades. In addition to their use in fundamental research, applications of practical importance have been established in some areas. However, no significant use of isothermal microcalorimetry has yet been seen in practical applications of biology, despite many methodological studies reported from that area. Th...

Five fucosylated glycoclusters exhibiting 4, 6 or 8 residues were synthesised with two different spatial environments based on mannose-centered and branched-phosphodiester scaffolds. Their synthesis was performed in solution using phosphoramidite chemistry to generate phosphodiester linkages, combined with Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC). The multivalent ligands were evaluate...

A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalori...

Isothermal titration microcalorimetry (ITC) is mostly used to investigate the thermodynamics of "specific" host-guest interactions in biology as well as in supramolecular chemistry. The aim of this review is to demonstrate that ITC can also provide useful information about non-specific interactions, like electrostatic or hydrophobic interactions. More attention will be given in the use of ITC t...

Number of Papers published in peer-reviewed journals: Number of Papers published in non peer-reviewed journals: Final Report: High Stability Metal-Protein Interactions Evaluated by Microcalorimetry Report Title Isothermal titration calorimetry (ITC) is a microcalorimetry technique used to study interactions between biomolecules and ligands in aqueous solution. This ARO equipment grant was provi...

effects of ?-cyclodextrin, ?cd, on refolding of lysozyme was investigated at ph 12 employing isothermal titration calorimetry (itc) at 300k in 30mm tris buffer solution. ?cd was employed as an anti-aggregation agent and the heats obtained for lysozyme+?cd interactions are reported and analyzed in terms of the extended solvation model. it was indicated that there are two sets of identical and no...

Abstract Background Neuroblastoma is the most common solid childhood tumor outside central nervous system. Metastatic and relapsed disease in neuroblastoma patients still has a poor prognosis. New treatment methods to improve therapy outcomes for with advanced are urgently needed. However, clinical trials currently only allow testing few substances even fewer patients. This increases need advan...