نتایج جستجو برای: isothermal titration
تعداد نتایج: 26326 فیلتر نتایج به سال:
Detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single Ig-binding domain of protein L from Peptostreptococcus magnus binding to kappa light chain are due to the binding of the kappa light chain at two separate...
The main calorimetric principles used in isothermal microcalorimetry are briefly discussed. Different chemical calibration and test reactions are discussed, with a focus on reactions suitable for ambient conditions: reactions initiated by mixing of liquids (including titration microcalorimetry), dissolution of solid compounds and of slightly soluble gases, a photochemical process, and thermal p...
To apply enzymes in technical processes, a detailed understanding of the molecular mechanisms is required. Kinetic and thermodynamic parameters enzyme catalysis are crucial to plan, model, implement biocatalytic processes more efficiently. While kinetic parameters, Km kcat, often accessible by optical methods, determination requires sophisticated methods. Isothermal titration calorimetry (ITC) ...
The effect of dextran sulfate on protein aggregation was investigated to provide the clues of its biochemical mechanism. The interaction between dextran sulfate and BSA varied with the pH values of the solution, which led to the different extent of aggregation prevention by dextran sulfate. Light scattering data with thermal scan showed that dextran sulfate suppressed BSA aggregation at pH 5.1 ...
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