This study aimed to investigate whether Opuntia spp-extracts protect against diclofenac (DF)-induced hepatotoxicity. Rats were pretreated with extracts, betanin (Bet) and N-acetylcysteine (NAC) followed by a single challenge of diclofenac. Liver tissue was collected for biochemical histological analysis. Primary rat hepatocytes treated (400 µmol/L) without pretreatment extract. Apoptosis measur...

Imatinib is an orally administered tyrosine kinase inhibitor which inhibits the Bcr-Abl protein-tyrosine kinase with high selectivity. Imatinib is rapidly absorbed from the gut, after oral intake and has an almost absolute bioavailability of 98%. The metabolism of imatinib is mediated by the cytochrome P450 (CYP) isoenzymes in the liver and gut wall. CGP74588 is a major active metabolite of ima...

Background: Mitochondria are a source of reactive oxygen species (ROS), and several natural compounds used as antioxidant agents. This study aimed to investigate compare the effects fresh grape juice red wine on oxidative stress biomarkers in rat liver mitochondria. Materials Methods: In this regard, mitochondria were isolated from 27 male Wistar rats (220-250 g). The cultured different doses f...

The disposition of mefloquine has been investigated in the isolated perfused rat liver (IPRL) preparation after the administration of [C]mefloquine HCI (3.8 mg, 4 1 Ci, quinoline ring labeled). Mefloquine underwent avid hepatic uptake within 10 min of dosing. Also at this point, hepatic oxygen consumption was reduced markedly in four of the six IPRL preparations, but was restored completely by ...

The ratios of total histone to DNA for rat liver nuclei isolated by four methods as well as for calf liver nuclei isolated by one method were determined by obtaining the ratios of the total areas of the electrophoretic histone peaks for the liver nuclei to the corresponding total area given by a known amount of standard calf thymus histone. Ratios of total histone to DNA of approx. 2 for rat li...

Amino acid incorporation by isolated intact rat liver mitochondria was severely inhibited by chloramphenicol, carbomycin, or sparsomycin, partially inhibited by emetine, and unatIected by erytbromycin or lincomycin. By contrast, amino acid incorporation by inner membrane-matrix fractions prepared from rat liver mitochondria by digitonin was strongly inhibited by emetine, erythromycin, and linco...

Rat lymph chylomicrons and chylomicron remnants were treated with trypsin or Pronase. The ability of the resulting apoprotein-free lipoproteins to be taken up by the isolated perfused rat liver, and to bind to isolated rat liver membranes, was examined. Compared with control lipoproteins, the apoprotein-free chylomicrons and remnants retained unaltered their capacity to be differentiated by the...

Arginase appears to be one of the first enzymes which was studied in cell nuclei isolated on a relatively large scale by physicochemical methods. This enzyme was measured in isolated nuclei of liver cells by Behrens (1) who reported that the arginase activity per dry weight of isolated nuclei was about equal to the arginase activity of cytoplasm per dry weight in the case of mammalian liver. It...