We have developed a new and simple flow cytometric method to detect damaged red blood cells (RBCs) using anti-Hb in hypotonic solution. We studied a total of 200 patients, including 62 patients with schistocytosis, 8 postsplenectomy patients, and 108 healthy controls. Peripheral blood (2 microl) was stained with phycoerythrin-conjugated (PE) antihemoglobin antibody (anti-Hb) in 0.6% (w/v) NaCl ...

PURPOSE Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is a member of the EGF family and plays a pivotal role in tumor progression in ovarian cancer. We developed an anti-HB-EGF monoclonal antibody (mAb) and investigated its antitumor activities in vitro and in vivo to evaluate its potential as a therapeutic antibody against ovarian cancer. EXPERIMENTAL DESIGN We pr...

OBJECTIVE The upregulated expression of heparin binding EGF-like growth factor (HB-EGF) in the vessel and circulation is associated with risk of cardiovascular disease. In this study, we tested the effects of HB-EGF targeting using HB-EGF-specific antisense oligonucleotide (ASO) on the development of aortic aneurysm in a mouse aneurysm model. APPROACH AND RESULTS Low-density lipoprotein recep...

Human hemoglobins (Hbs) are known to be immunogenic, and both normal and variant forms of Hb have been shown to stimulate antibody formation in a variety of animal species. In patients who are homozygous for the sickle Hb (HbS) mutation, transfusion of normal, HbA-containing erythrocytes provides a potential stimulus for HbA alloimmunization. We tested serum samples for the presence of anti-Hb ...

BACKGROUND Whether hepatitis B (HB) vaccine-conferred immunity persists into adulthood is unknown. We aimed to investigate long-term HB immunity in adolescents. METHODS In 2004-2005, 6156 high school students (15-21 years old) who had been vaccinated with plasma-derived HB vaccine as infants were recruited for HB seromarker screening. The immune response to an HB vaccine booster was evaluated...

By TITUS H. J. HuIS sL&rc AND RALPH C. LEE I N A PREVIOUS PAPER6 we reported the discovery of an abnormal minor hemoglobin in a Negro family ( Fam. S ) in Georgia. This hemoglobin, designated as Hb-Flatbush0 , showed the following properties : ( a ) an electrophoretic mobility ( starch gel electrophoresis at pH 8.1 ) intermediate between Hb-S and Hb-F; ( b ) a chromatographic mobility ( DEAE-ce...

Background/aim: Immunogenicity and safety of a primary series of a fully liquid, hexavalent DTaP-IPV-HB-PRP-T vaccine given at 2, 3, and 4 months of age compared to licensed comparators and a DTaP-IPV-HB-PRP-T booster at 15?18 months were evaluated. Materials and methods: This was a Phase III, randomized, open-label trial. Primary series (no hepatitis B [HB] at birth) of DTaP-IPV-HB-PRP-T (N = ...

objective: preventing communicable diseases in the armed forces (af) of a country is considered as a national interest and has a great importance. there are limited data on the efficacy of vaccination of the navy personnel. this study was designed to evaluate both the safety and the immunogenicity of a recombinant hepatitis b (hb) vaccine made by pasteur institute of iran (ipi-rhb), in this tar...

The aim was to study the association of CD150 expression in peripheral blood mononuclear cells (PBMCs) with response to hepatitis B (HB) vaccination. Heparinized blood drawn from non-responders and responders was used to obtain PBMCs. Out of 460 adult healthy males and non-pregnant females, 27 subjects who were negative for HB markers were defined as non-responders (15 males and 12 females, age...

conclusions the study results indicated that the neonatal hbv immunization induced a long-term protection against hbv and was very efficacious in reducing chronic hbv infection rate in vaccinated young adults in iran. results anti-hbs seropositivity rate was detected in 224 of 510 [95% ci: 39-47] young adults. breakthrough infection (positive sera for anti-hbc without chronic infection) was obs...