A new isocratic HPLC method is optimized and validated for simultaneous determination of Vildagliptin (VLD), Pioglitazone Hydrochloride (PIO) and Glimepiride (GLIM) in Bulk and tablets (Gliptus ® and Amaglust ® tablets). The chromatographic separation was achieved on a reversed-phase analytical column [Hypersilgold ® C18 (10μm, 150 x 4.6 mm) column] at ambient temperature. The separation was ac...

The separation of dilute binary mixtures of proteins by salt aided ion-exchange simulated moving bed (SMB) chromatography is optimized with respect to throughput, desorbent consumption and salt consumption. The optimal flow-rate ratios are analytically determined via an adopted "triangle theory". Azeotropic phenomena are included in this procedure. The salt concentrations in the feed and recycl...

A simple, precise, and accurate isocratic RP-UFLC stability-indicating assay method has been developed to determine trospium chloride in tablet dosage form. Isocratic separation was achieved on an Enable-C18G (250 mm × 4.6 mm i.d., particle size 5 μm) column at room temperature, the mobile phase consisted of acetonitrile:0.01M TBAHS (50:50, v/v) at a flow rate of 1.0 ml/min, the injection volum...

A simple, precise, and accurate isocratic reversed-phase (RP) stability-indicating HPLC assay method was developed and validated for determination of Aripiprazole in bulk and solid pharmaceutical dosage form. A reversed-phase C8 (250×4.0 mm, 5 μm particle size) column for HPLC and C8 (50×2.1mm, 1.7 μm particle size) for UPLC method in isocratic mode was used. The mobile phase consists of aceton...

A simple, selective and precise stabilityindicating HPLC method for determination of glabridin diacetate and dihexanoate prodrugs was developed, validated and applied to the enzymatic and chemical hydrolysis studies. The chromatographic separation was achieved on a reverse phase C18 (Thermo Hypersil-Keystone, 250 × 4.6 mm, 5 micron) column using the mixture of acetonitrile and water as mobile p...

In reversed-phase liquid chromatography (RPLC), the selectivity between major species and minor variants of protein biopharmaceutical products is always limited. Unfortunately, stationary phase chemistry, type mobile (organic modifier salts) temperature only have a very limited impact on selectivity. Therefore, instead using linear elution gradient, we evaluated recently developed strategy, nam...