the efficacy of bacterial cultures and is900-specific polymerase chain reaction (pcr) was compared for the detection of mycobacterium avium subsp. paratuberculosis (map) from the mesenteric lymph nodes of goats. samples were collected from 75 goats slaughtered in ilam, in southwest of iran. tissue homogenates were inoculated onto four media. the genomic dna was extracted directly from mesenteri...

Mycobacterium avium subsp paratuberculosis was isolated from two out of seventy samples (2.86 %) of pasteurized and ultra-pasteurized milk. The isolates were positives to IS900 PCR and showed a C17 RFLP pattern, the most prevalent in Argentina. The present study is the first report of Mycobacterium avium subsp paratuberculosis culture from pasteurized milk in Argentina.

We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.

The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical signs of the disease. Based on clinical signs, in culture and in IS900 PCR results from the individual milk samples it was possible to diagnose paratuberculosis in the cows studied.

INTRODUCTION Johne’s disease (JD) is a serious infection of animals caused by Mycobacterium avium subspecies paratuberculosis (MAP). Johne’s is not a priority in India. Therefore, information on prevalence in 465.50 million domestic ruminants have not been estimated nor realized, mainly due to lack of indigenous diagnostic kits. Diagnosis is difficult due to long incubation, absence of characte...

Multitarget genotyping of the etiologic agent Mycobacterium avium subsp. paratuberculosis is necessary for epidemiological tracing of paratuberculosis (Johne's disease). The study was undertaken to assess the informative value of different typing techniques and individual genome markers by investigation of M. avium subsp. paratuberculosis transmission between wild-living red deer and farmed cat...

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains...

Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. C...

Figure 1. Representative PCR results showing amplification (or otherwise) of 298-bp genomic fragment corresponding to IS900 in diabetic patients (lanes 1–8). Lanes 9 and 10, Control subjects without type 1 diabetes mellitus. Lanes 11 and 12, Mycobacterium avium subspecies paratuberculosis–positive and M. avium subspecies paratuberculosis–negative control subjects, respectively. M, molecular mar...