The end sequences of the IS50 insertion sequence are known as the outside end (OE) and inside end. These complex ends are related but nonidentical 19-bp sequences that serve as substrates for the activity of the Tn5 transposase. Besides providing the binding site of the transposase, the end sequences of a transposon contain additional types of information necessary for transposition. These addi...

Challenges Facing Industrial Analytical Labs Many routine QC/QA laboratories can perform material analyses with single range, basic Fourier transforminfrared (FT-IR) instrument configurations. However, modern analytical laboratories face increasing workloads from a broad range of sample types with a simultaneous drive for faster results and more complex sample characterization needs. Flexibilit...

Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fashion through the action of the element-encoded transposase protein and the cl...

Infrared (IR) spectroscopy is used extensively to characterize the chemical composition of trace evidence such as paints, fibers and adhesives as well as seized drugs and related chemicals. We have recently introduced the Thermo ScientificTM iSTM50 ATR module, a novel, extended-range attenuated total reflectance (ATR) module that is integrated into the NicoletTM iS50 FT-IR spectrometer. This in...