Capecitabine (Xeloda; CAP) is a recently developed oral antineoplastic prodrug of 5-fluorouracil (5-FU) with enhanced tumor selectivity. Previous studies have shown that CAP activation follows a pathway with three enzymatic steps and two intermediary metabolites, 5 -deoxy-5-fluorocytidine (5 -DFCR) and 5 -deoxy-5-fluorouridine (5 -DFUR), to form 5-FU preferentially in tumor tissues. In the pres...

Introduction: To expand our ability to test current concepts about system and organ function within organisms in normal and disease states we need a new class of discrete, event-driven simulation models that achieve a higher level of biological realism across multiple scales, while being sufficiently flexible to represent different aspects of the biology. Here we provide the first description o...

The catabolism <>l5-fluorouracM ill mi »as measured in isolated perfused rat liver (IPRL) at various times of the day. IPRLs were prepared from rats sacrificed at 3-h intervals and the elimination rate of FUra and FUra catabolites (i.e., rate leaving the II'KI in the effluent perfusate) following infusion of |'I l|l I ni »as analy/.ed for circadian periodicity. Animals were housed under stand...

Hypothermia may attenuate the progression of ischemia-induced damage in liver. Here, we determined the effects of a brief cycle of hypothermic preconditioning applied before an ischemic/reperfusion (I/R) episode in isolated perfused rat liver (IPRL) on tissue damage and oxidative stress. Rats (male, 200-250 g) were anaesthetised with sodium pentobarbital (60 mg·kg-1 i.p) and underwent laparatom...

Colchicine-specific goat IgG and Fab fragments were cationized by covalent coupling of hexamethylenediamine. The immunoreactivity of antibodies was not changed following cationization. The interaction of 125I-radiolabeled native (nIgG and nFab) and cationized immunoglobulin G (cIgG) and Fab fragments (cFab) with liver was investigated using isolated perfused rat liver (IPRL) and isolated rat he...