introduction: the acrosome is a sac shaped organelle contaning lysosomal enzyme that is located on the anterior of sperm head. during the acrosome reaction its content is released in the vicinity of the egg. the acrosome reacted sperm then penetrates through the egg barriers. there are several methods for evaluating the acrosomal status; some of those are complex, expensive and/or non-accurate ...

The electrochemical ion-sensor performance of the self-assembled monolayer (SAM) 3H-imidazo[4,5-b]pyridine, which is known as a metal ionophore, was examined using triad composed ferrocene, an and tripodal trithiol with adamantane core. voltammetry SAM-modified Au electrode showed large positive change (280 mV) in ferrocene oxidation potential via addition Pb2+, detection limit 10–6 M. A Langmu...

Abstract The decentralisation of accurate determination the ammonium ion (NH 4 + ) is relevant for environmental monitoring (i. e., nitrogen cycle) and certain clinical applications (e. g., kidney liver diseases). Potentiometric ionophore‐based sensors are one alternative these purposes in terms versatile implementation, though potassium (K known to be a major source interference. We herein inv...

Background The role of calcium in physiological phenomenona such as sperm acrosome reaction and hyper-activation has been well known. Calcium ionophore A23187 is routinely used for induction of acrosome reaction in mammalians sperm. Therefore, we decided to evaluate the effect of calcium ionophore A23187 on the sperm hyper-active motility patterns. MaterialsAndMethods Ten semen samples from ind...

The addition of the Ca2+ ionophore A23187 to rabbit neutrophils stimulated [14C]arachidonic acid incorporation into phosphatidylinositol and lysosomal enzyme secretion. A significant increase in phosphatidylinositol labelling was observed after a 2 min exposure to 0.1 microM-ionophore A23187. Maximum increases in rate of labelling were obtained with 1 microM-ionophore A23187 within 1 min, decli...

This study was designated to clarify the influence of activation of porcine matured oocytes by calcium ionophore on in vitro development of the parthenotes. The follicular oocytes were matured, activated and cultured in North Carolina State University-23 (NCSU-23) medium supplemented with 10% porcine follicular fluid (pFF). The in vitro-matured oocytes were exposed to calcium ionophore at conce...

The processes involved in transport of K and Na by the carrier ionophores valinomycin and 18-crown-6 ether across cell membranes have been elucidated using quantum mechanical modelling: 1. Formation of the {ionophore-M} complex: desolvation (ΔGdesolv) of the central cavity of the ionophore, change in configurational energy TΔS, desolvation of the M(H2O)6-7 + 2. Desolvation of the {ionophore-M} ...

Ca2+ has been recently reported to be required for high rates of translational initiation in GH3 pituitary cells (Chin, K.-V., Cade, C., Brostrom, C.O., Galuska, E.M., and Brostrom, M.A. (1987) J. Biol. Chem. 262, 16509-16514). In the present investigation low concentrations of the Ca2+ ionophores, A23187 and ionomycin, were found to rapidly suppress the Ca2+-dependent component of protein synt...

BACKGROUND Due to the increasing industrial use of titanium compounds, its determination is the subject of considerable efforts. The ionophore or membrane active recognition is the most important component of any polymeric membrane sensor. The sensor's response depends on the ionophore and bonding between the ionophore and the target ion. Ionophores with molecule-sized dimensions containing cav...