Both specific polyclonal antiserum and monoclonal antibodies against mannose-binding hemagglutinin fimbriae of Vibrio cholerae (mannose-sensitive hemagglutinin [MSHA]) were shown to protect against experimental cholera caused by vibrios of the El Tor biotype in the infant mouse and in the rabbit intestinal loop models. MSHA-specific Fab immunoglobulin fragments were also protective. No protecti...

We have investigated the interactions of the plant toxin ricin with sinusoidal endothelial rat liver cells (EC). In these cells, ricin can be bound and internalized via either cell surface galactosyl residues or mannose receptors. Binding and uptake via galactosyl residues and mannose receptors was studied in the presence of mannan (1 mg/ml) and lactose (50 mM) respectively. Whereas most of the...

Proliferin is a prolactin-related glycoprotein secreted by proliferating mouse cell lines and by mouse placenta. In an attempt to identify target sites for proliferin action, we looked for proliferin receptors in murine fetal and maternal tissues during pregnancy using proliferin purified from the conditioned medium of a constructed Chinese hamster ovary cell line carrying amplified copies of p...

Adsorptive endocytosis of alpha-N-acetylglucosaminidase from human urine by isolated rat hepatocytes is inhibited by glycoproteins, polysaccharides and sugars that are known to bind to cell-surface receptors specific for either terminal galactose/N-acetylgalactosamine residues, terminal mannose residues or mannose 6-phosphate residues. Recognition of alpha-N-acetylglucosaminidase by a cell-surf...

We have purified phosphomannosyl-enzyme receptors from bovine liver on an affinity column composed of glycoproteins isolated from Dictyostelium discoideum secretions. Binding of human fibroblast beta-hexosaminidase B to receptors reconstituted into phosphatidylcholine liposomes was 1) specifically inhibited by mannose 6-phosphate, but not mannose 1-phosphate or glucose 6-phosphate, and 2) had p...

Flow cytometry was used to quantify the abundance of mannose-linked glycoconjugates on microalgae precultured using low- or high-nitrate media. Nitrogen-deficient microalgae were richer in cell-surface mannose than nitrogen-sufficient. Findings are discussed in view of recent research which reveals mannose-specific 'feeding receptors' assist prey biorecognition by phagotrophic protozoa that ing...

Tuberculosis (TB) is a chronic infectious disease mainly caused by Mycobacterium tuberculosis (M. tuberculosis). The structures of polysaccharides and glycolipids at M. cell wall vary among different strains, which affect the physiology pathogenesis mycobacteria activating or inhibiting innate acquired immunity. Among them, some components such as lipomannan (LM) lipoarabinomannan (LAM) activat...

Concanavlin A (ConA), a lectin found in jack-beans can bind to multiple sugars, as can cell receptors found on cheek epithelial cells and erythrocytes. By using horseradish peroxidase and hemagglutination, the effects of mannose and galactose on ConA binding were observed. Galactose had no effect on ConA but mannose bound with more affinity to ConA, competed negatively with the cell receptors, ...

Background: Vasoactive intestinal peptide (VIP) is an important neurotransmitter involved in the modulation of gastrointestinal function through stimulation VIP receptors. However, expression VPAC1R, VPAC2R and PAC1R human Lower esophageal sphincter (LES) has not been fully clarified. Therefore, purpose this study to explore these receptors sphincter, responses peptide, role responses.

We examined the carbohydrate-binding potential of the C-type lectin-like receptor Dectin-2 (Clecf4n). The carbohydrate-recognition domain (CRD) of Dectin-2 exhibited cation-dependent mannose/fucose-like lectin activity, with an IC(50) for mannose of approximately 20 mM compared to an IC(50) of 1.5 mM for the macrophage mannose receptor when assayed by similar methodology. The extracellular doma...