Chromatin insulators are DNA protein complexes situated throughout the genome capable of demarcating independent transcriptional domains. Previous studies point to an important role for RNA in gypsy chromatin insulator function in Drosophila; however, the identity of these putative insulator-associated RNAs is not currently known. Here we utilize RNA-immunoprecipitation and high throughput sequ...

Sandstorm has an effect on the electric field characteristics of outdoor insulator, and may result in flashover. The analysis of the electric field characteristic of insulator under sandstorm condition is very important. The electrostatic field finite element method (FEM) is used to calculate the electric field distribution along long rod insulator under sandstorm condition with FEM software. T...

We propose a closed form formulation for the impedance of the metal-insulator-metal (MIM) plasmonic transmission lines by solving the Maxwell's equations. We provide approximations for thin and thick insulator layers sandwiched between metallic layers. In the case of very thin dielectric layer, the surface waves on both interfaces are strongly coupled resulting in an almost linear dependence of...

Chromatin insulators are DNA-protein complexes that are situated throughout the genome that are proposed to contribute to higher-order organization and demarcation into distinct transcriptional domains. Mounting evidence in different species implicates RNA and RNA-binding proteins as regulators of chromatin insulator activities. Here, we identify the Drosophila hnRNP M homolog Rumpelstiltskin (...

Chromatin insulators have been implicated in the establishment of independent gene expression domains and in the nuclear organization of chromatin. Post-translational modification of proteins by Small Ubiquitin-like Modifier (SUMO) has been reported to regulate their activity and subnuclear localization. We present evidence suggesting that two protein components of the gypsy chromatin insulator...

Chromatin insulators are thought to regulate gene expression by establishing higher-order domains of chromatin organization, although the specific mechanisms by which these sequences affect enhancer-promoter interactions are not well understood. Here we show that the gypsy insulator of Drosophila can affect chromatin structure. The insulator itself contains several DNase I hypersensitive sites ...

Through this paper work we have analyzed the effect of different contaminants on the flashover by creating artificial pollution condition, considering the natural condition where more than one salt as contaminant deposits on the surface of insulator at a time, under the wet condition that cause flow of highly leakage current, that leads to flashover. We choses the mixture of different salts, so...

The Drosophila gypsy retrotransposon disrupts gene activity by blocking the interactions of distal enhancers with target promoters. This enhancer-blocking activity is mediated by a 340 bp insulator DNA within gypsy. The insulator contains a cluster of binding sites for a zinc finger protein, suppressor of Hairy wing [su(Hw)]. Recent studies have shown that a second protein, mod(mdg4), is also i...

Insulator elements mediate intra- and inter-chromosomal interactions. The insulator protein CCCTC-binding factor (CTCF) is important for insulator function in several animals but a report in BMC Molecular Biology shows that Caenorhabditis elegans, yeast and plants lack CTCF. Alternative proteins may have a similar function in these organisms.