نتایج جستجو برای: insoluble residue

تعداد نتایج: 62165  

Journal: :Zeitschrift fur Naturforschung. Section C, Biosciences 1982
K Mann D Mecke

Saccharomyces cerevisiae nuclear membranes were prepared from isolated nuclei by digesting chromatin with deoxyribonuclease after an initial treatment of nuclei with very diluted buffers. When the nuclear membranes were treated with 5% Triton X-100 and 1M NaCl an insoluble fibrous net was obtained which consisted mainly of protein with Mr values of 85 000, 48 000, 45 000, 39 000 and 31 000. Lam...

Journal: :Journal of the American Chemical Society 2012
Jing Xu Jin Wang J Christopher Luft Shaomin Tian Gary Owens Ashish A Pandya Peter Berglund Patrick Pohlhaus Benjamin W Maynor Jonathan Smith Bolyn Hubby Mary E Napier Joseph M DeSimone

Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker lea...

Journal: :Journal of bacteriology 1997
S Chen L Zheng D R Dean H Zalkin

Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11-residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature enzyme forms as well as a small fracti...

2005
Murray R. GRAY Janet A. W. ELLIOTT William C. MCCAFFREY

The vacuum residue fraction of petroleum, comprising material with a normal boiling point of over 524°C, is a complex mixture of components with a wide range of molecular weight and chemical structure. The emphasis in process development and design for conversion of the vacuum residue fraction has tended to focus on the chemistry of the feed and the cracked products, and to some extent the chem...

Journal: :Molecules 2012
Bo Li Fei Lu Haijuan Nan Yang Liu

Okara is a byproduct generated during tofu or soymilk production processes. Crude polysaccharide (yield 56.8%) was isolated by removing fat, protein and low molecular weight carbohydrates from initial okara. Crude okara polysaccharide was further divided into four soluble fractions and an insoluble residue fraction by extracting with 0.05 M EDTA + NH(4) oxalate, 0.05 M NaOH, 1 M NaOH and 4 M Na...

Journal: :The Journal of general virology 1984
R F Marsh B E Castle C Dees W F Wade

Plasma membrane-enriched preparations from scrapie-infected and healthy hamster brains were detergent-extracted, then separated by equilibrium density centrifugation in continuous Nycodenz gradients. The highest level of infectivity was always associated with the insoluble residue which sedimented through 40% Nycodenz. The degree of aggregation in these insoluble complexes varied depending upon...

2013
Hua-Min Liu Ming-Fei Li Sheng Yang Run-Cang Sun

The mechanism of hydrothermal liquefaction of cypress was investigated by examining the effects of temperature and retention time on the characteristics of the solid residues remaining after liquefaction. The solid residues were divided into acid-soluble and acid-insoluble residues. Results showed the polymerization reactions also mainly occurred at low temperatures. The reactive fragments tran...

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