Production of oxytetracycline by Streptomyces rimosus in several chemically defined media containing graded concentrations of inorganic phosphorus was studied in shake flasks. Although high levels of inorganic phosphate have been reported to inhibit oxytetracycline formation, this study indicated that composition of the medium is an important factor in determining whether antibiotic production ...

31P NMR spectra of the human heart are usually contaminated by signals that originate from blood. The main blood signals are 2,3-diphosphoglycerate (2,3-DPG), which overlap and sometimes obscure the signal of myocardial inorganic phosphate used to calculate intracellular pH and to monitor metabolic changes in the heart. In this work we demonstrate, first, that even without proton decoupling the...

Inorganic phosphate must be transported continuously into the mitochondrial matrix to maintain the steady-state oxidative phosphorylation of ADP. Specific uptake of this anion occurs via at least two distinct transport systems: an electroneutral inorganic phosphate-dicarboxylic acid exchange carrier and an inorganic phosphate-hydroxyl antiporter which catalyses the influx of about 90% of the mi...

Esterification of inorganic phosphate accompanies the oxidation of succinic acid by cell-free extracts of E. coli. Inorganic phosphate appears to be an essential requirement for the dehydrogenation of the C(4)-dicarboxylic acid.

A phosphorylated derivative of streptomycin accumulated in cultures of Streptomyces griseus ATCC 12475 and SC2376 grown on complex media containing an excess of inorganic phosphate (0.01 m). This compound did not accumulate significantly in the absence of added inorganic phosphate. The phosphorylated derivative did not inhibit growth of Bacillus subtilis or support growth of a streptomycin-depe...

W7hen a resting skeletal muscle is treated with cold perchloric or other acid extracting agent, and the solution is analyzed with one of the standard methods for inorganic phosphate, values are obtained that differ for various kinds of muscle, but have been reported as about 5 pmoles per g for the sartorius muscle of the frog (I; see also Tables IV and V). For several reasons, we have felt the ...

The effects of increased concentration of inorganic phosphate on the biosynthesis of tylosin, the level of the intracellular adenylates, the energy charge, and the activities of enzymes involved in the synthesis of tylonolide precursors were studied in Streptomyces fradiae NRRL 2702. No metabolic response was observed when elevated levels of inorganic phosphate were added in idiophase. Increase...

AIM To assess the potential of acute alcohol consumption to dissolve tooth surfaces and to evaluate the difference in the dissolution potential of whisky, beer and wine. METHODS The study sample comprised 36 healthy male volunteers with mean age of 26.27 (SD-1.96) years (range 25-30 years). The study design involved randomly allocating the 36 individuals into three groups of alcohol consumpti...