SOME years ago during the progress of an inquiry on the production of an antiscorbutically active cider from Bramley's Seedling apples it was observed that the juice expressed from the fruit while retaining a very high proportion of its antiscorbutic activity did not reduce indophenol. At that time the writer held the view that the capacity for reducing this indicator was mainly due to a substa...

We developed a method for the selective determination of 4-aminobenzoic acid by use of 4-aminobenzoate hydroxylase (EC 1.14.13.27) to convert 4-aminobenzoic acid to 4-hydroxyaniline. The subsequent conversion of 4-hydroxyaniline to indophenol dye was quantified colorimetrically. The method is reproducible, and the assay response is linear up to 40 mumol/L. Selectivity exceeding that of the curr...

Simplified methods were described for the measurement of growth of human car cinoma cells in tissue culture: (a) determination of color intensity in lysates of cells stained with crystal violet (CV method), (6) determination of the reduction of 2,6dichlorophenol indophenol, and (c) determination of total organic solids (dichromate method). The three methods gave comparable measurements of relat...

in this work, an optical sensor for detection of ascorbic acid is described. the sensor is fabricated by casting and spin-coating silica sol-gel doped with 2,6-dichlorophenol indophenol on the glass plates. the sols are in turn obtained by acidic hydrolysis of various precursors such as tetraethoxysilane, triethoxymethylsilane and triethoxyoctylsilane in the presence of 2,6-dichlorophenol indop...

Adenosine is a major local regulator of tissue function and industrially useful as precursor for the production of medicinal nucleoside substances. High-throughput screening of adenosine overproducers is important for industrial microorganism breeding. An enzymatic assay of adenosine was developed by combined adenosine deaminase (ADA) with indophenol method. The ADA catalyzes the cleavage of ad...