Flow cytometry can be used to detect bacterial contamination of platelet products. In this study, we investigated whether the incubation of a minimal volume of platelet-rich plasma (PRP)-derived platelet concentrates (PCs) with growth medium improved the analytical sensitivity of flow cytometry. Five bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Klebsiel...

background: control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. objective: the objectives of the present study were to find the effects of different osmolarities of modified tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. materials and methods: semen samples were collected twice from five najdi bu...

MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3'-end length variants ("isomiRs"), little attention has been paid to their differential detection by RT-qPCR. However, recent evidence indicates that 3'-end miRNA i...

--Bird eggs begin to lose weight as soon as they are laid but their volume and linear dimensions do not change during incubation. The volume of an egg can be estimated within 2% from the relationship: Volume = 0.51. LB2, where L is the length and B is the breadth (maximum diameter). The fresh weight of an egg can be estimated within 2% from the relationship: Weight = Kw' LB 2, where K w is a sp...

to investigate the influence of hypoxia during the early stage of incubation on embryonic developmentand hatching events, and consequently on incidence of ascites in broiler chickens, one thousand fertile eggswere incubated in two commercial incubators. half the eggs were incubated in a low altitude incubator untilhatched. the second half were incubated in a high altitude incubator until day 10...

Campylobacter jejuni is a pathogenic, microaerophilic, gram-negative, mesophilic bacterium. Three strains isolated from humans with enteric campylobacteriosis were able to survive at high population levels (10(7) cells ml-1) as viable-but-nonculturable (VBNC) forms in microcosm water. The VBNC forms of the three C. jejuni strains were enumerated and characterized by using 5-cyano-2,3-ditolyl te...

knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old ece...