This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

Zona pellucida (ZP) hardening (resistance to proteolysis) has been classically identified as a post-fertilization event that contributes to the block to polyspermy. Di-(N-succinimidyl)-3,3'-dithiodipropionate (DSP), a permeable amine-reactive cross-linker, was recently shown to induce pre-fertilization ZP hardening and to improve porcine IVF productivity. The objectives of this study were to in...

background: assisted reproductive technologies (art) are known as a treatment for infertility in which oocyte undergoes manipulation outside body. the aim of this study was to study the evaluation of birth defects in infants conceived through assisted reproductive technologies (art) and compare them with naturally-conceived infants. materials and methods: in this retrospective study, four hundr...

a study was conducted to investigate the effect of follicular size (small,

The aim of this study was to compare the effect of the addition of follicular fluid (FF) collected from preovulatory follicles with that of oestrous mare serum (EMS) (acting as the control) to TCM-199 medium on the in-vitro maturation, fertilization and development of equine cumulus-enclosed oocytes. Oocytes (<30 mm in diameter) were obtained from the ovaries of slaughtered mares. After in-vitr...

Sperm cryopreservation has been widely adopted for maintenance of the genetically engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes worldwide. However, the recipients are not always able to obtain high fertilization rates with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm via various methods and performed in vitro fert...

this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...

BACKGROUND/AIMS The importance of sperm capacitation for mammalian fertilization has been confirmed in the present study via sperm metabolism. Involvement of the metabolic enzymes pyruvate dehydrogenase complex (PDHc) and its E3 subunit, dihydrolipoamide dehydrogenase (DLD) in hamster in vitro fertilization (IVF) via in vitro sperm capacitation is being proposed through regulation of sperm intr...

introduction: standard semen analysis is routinly used to evaluate the male fertility, however some researchers belive that sperm functional test should also be used with standard semen analysis for more precise evaluation of male fertility. the aim of this study was to consider the relation between different nuclear maturity tests and in vitro fertilization, in order to select the nuclear matu...