this study was conducted to determine the chemical composition, digestibility, degradability and nutritive value of madder, rubia tinctorum l.  and alfalfa medicago sativa. using in vitro and in situ measurements. the mean values for om, cp, ndf, adf and ee were 892.5, 172.0, 306.5, 219.4 and 16.0 g/kg dm for madder hay, and 921.3, 141.4, 482.0, 361.1 and 6.50 g/kg dm for alfalfa hay, respectiv...

influenza c virus possesses specific neuraminate-o-acetylesterase as a receptor-destroying function. this enzymatic activity of the viral glycoprotein hef (hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. hereby the localization, as well as the quantity of synthesized hef protein is detectable. we further developed the estera...

Intracellular vesicle transport defects can induce retinal degeneration and photoreceptor cell death, but the molecular connections between these processes remains poorly understood. Reporting in Developmental Cell, Nishiwaki et al. (2013) suggest that a vesicle fusion cis-SNARE complex component translates vesicular transport defects into photoreceptor cell apoptosis.

To process large numbers of samples in parallel is one potential of protein microarrays for research and diagnostics. However, the application of protein arrays is currently hampered by the lack of comprehensive technological knowledge about the suitability of 2-D and 3-D slide surface coatings. We have performed a systematic study to analyze how both surface types perform in combination with d...

At physiological protein levels, the slow HOPS- and SNARE-dependent fusion which occurs upon complete SNARE zippering is stimulated by Sec17 and Sec18:ATP without requiring ATP hydrolysis. To stimulate, Sec17 needs its central residues which bind the 0-layer of the SNARE complex and its N-terminal apolar loop. Adding a transmembrane anchor to the N-terminus of Sec17 bypasses this requirement fo...

The homotypic fusion of yeast vacuoles requires Sec18p (NSF)-driven priming to allow vacuole docking, but the mechanism that links priming and docking is unknown. We find that a large multisubunit protein called the Vam2/6p complex is bound to cis-paired SNAP receptors (SNAREs) on isolated vacuoles. This association of the Vam2/6p complex with the cis-SNARE complex is disrupted during priming. ...

In neurons, the establishment and maintenance of distinct somatic, dendritic, and axonal domains has long been known to rely on regulated traffic of organelles and proteins. More recently, the local targeting of specific mRNAs has also been demonstrated, at least in dendrites, to provide a local supply of specific proteins. Here we set out to test directly for the presence of mRNA in axons of c...

Fluorescence in situ hybridization (FISH) is a macromolecule recognition technique, which is considered as a new advent in the field of cytology. Initially, it was developed as a physical mapping tool to delineate genes within chromosomes. The accuracy and versatility of FISH were subsequently capitalized upon in biological and medical research. This visually appealing technique provides an int...