Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza virus because the protocol is short time and easy to use. Despite the usability of IC, the sensitivity is approximately 10(3) pfu per reaction. In addition, antigen-antibody interaction-based method cannot be used for the detection of influenza viruses with major antigenic ch...

the present study was conducted to detect prevalence and risk factors for retrovirus infection of infected cats in a cat population in iran, by evaluation of 238 client-owned cats of different ages that were tested for the presence of fiv antibodies. the cats were selected from those referring to veterinary hospital of ahvaz university, southwestern iran from december 2007 to june 2010. classif...

Improvement of the methods included in the World Organisation for Animal Health (OIE) Manual of Diagnostic Tests for Aquatic Animals and addition of novel techniques are dependent on the continual development and evaluation of both new and existing methods. Although conventional isolation and characterisation techniques for the diagnosis of many diseases still remain the methods of choice there...

One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serol...

the present study was carried out for the antigen detection of feline panleukopenia virus (fpv) in diarrhoeic cats referred to the veterinary clinic of the school of ahvaz university, in southwest iran. faecal samples were collected from 67 diarrhoeic household cats during 2005 to 2007. according to the age and clinical signs, the cats were divided into two groups; 6 months, hemorrhagic and ...

background: acute gastroenteritis is responsible observed in all age groups, especially infants and children. the etiology and clinical course of acute gastroenteritis may vary with age and etiological agents. in developing countries, the morbidity and mortality associated with infectious diarrhea is higher in children younger than five-years-of-age. objectives: the aim of this study was to det...

Membrane-based rapid test reagents including immunochromatography are widely used in clinical practice. Recently, high-sensitive based on the method, such as silver amplification method and time resolved fluorescence for influenza testing, has been developed early confirmation of infection can be achieved. Furthermore, genetic automated all steps from extraction till detection, is getting popul...

Cannabis sativa L. is one of the most consumed drugs in world and recent studies have associated its use with an increase number traffic accidents different countries. In many countries, like Brazil, simple reliable methodologies are still needed for detection on site, mainly cannabinoids, considering prevalence oral fluid (OF) has been proved as appropriate biological matrix this purpose. Cons...

Immunodetection of methicillin-resistant Staphylococcus aureus (MRSA) by conventional methods employing mammalian immunoglobulins has unknown detection limits, and often yields false-positive results because of the presence of S. aureus protein A, which binds the Fc region of mammalian IgG. In this study, a new PBP2a-specific chicken IgY antibody was developed in inbred and conventional chicken...

The recent Zika virus (ZIKV) outbreak demonstrates that cost-effective clinical diagnostics are urgently needed to detect and distinguish viral infections to improve patient care. Unlike dengue virus (DENV), ZIKV infections during pregnancy correlate with severe birth defects, including microcephaly and neurological disorders. Because ZIKV and DENV are related flaviviruses, their homologous pro...