Through the use of specific immunoadsorbent columns, it is shown that Escherichia coli aspartokinase I-homoserine dehydrogenase I, aspartokinase II-homoserine dehydrogenase II, aspartokinase III, and homoserine kinase, enzymes involved in the same complex biosynthetic pathway, share antigenic determinants. This raises the question of a common origin for the four cibtenoirart kinases. (Aspartate...

Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human gamma-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used...

Ecdysteroid, Immunoadsorption of Steroids, Antiserum Specificity Two ecdysteroid-specific antisera, anti-ecdysone-albumin and anti-20-hydroxyecdysone-albumin, were induced in rabbits. The properties of both antisera were tested with a large number of ecdysteroids. The former antiserum was unique in its ability to discriminate between the moulting hormones, ecdysone and 20-hydroxyecdysone, where...

1. A rapid method for the isolation of hormonally sensitive rat fat-cell plasma membranes was developed by using immunological techniques. 2. Rabbit anti-(rat erythrocyte) sera were raised and shown to cross-react with isolated rat fat-cells. 3. Isolated rat fat-cells were coated with rabbit anti-(rat erythrocyte) antibodies, homogenized and the homogenate made to react with an immunoadsorbent ...

The Schu-5 strain of Pasteurella tularensis was cross-linked with tetra-azotized benzidine and employed for the isolation of purified specific antibodies. The isolation procedure increased the content of specifically precipitable antibodies at least 40-fold.

To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the ...