Objective: The aims of the present study were to screen actinobacteria with high potential ability produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production was analysis by thin-layer chromatography and bioautography assay.
 Methods: Screening selection Streptomyces sp. morphological, cultural, physiological, biochemical char...

Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, minerals. They significantly vulnerable, however, to post-harvest diseases caused by numerous fungal bacterial pathogens. These pathogens can cause significant quantitative qualitative losses from harvest consumption during the handling storage processes. Chemical fungicides commonly u...

A new bioassay has been developed combining the simplicity of direct bioautography with the improved chromatographic resolution of 2D-TLC. Mixtures of structurally diverse antifungal agents were tested to establish the validity and utility of this method in the discovery of new natural products with activity against agriculturally important fungal pathogens.

The use of high-voltage electrophoresis and bioautography for the separation mixtures of antibiotics received in clinical serum samples is described. The electrophoretic characteristics of 39 antibiotics are given as is the accuracy of the method for quantitating three antibiotics. In screening 189 consecutive serum samples submitted for routine antibiotic assay, undisclosed antibiotics were r...

A microbioautographic method for the identification of pteroylglutamic acid derivatives and their glutamyl conjugates is described. Chromatograms or pherograms of mixtures of the compounds are treated with chicken pancreas conjugase, followed by tetrazolium bioautography. This combined procedure allows qualitative and semi-quantitative analyses of folate compounds in biological material in the ...

Objective: The objective of the study was to carry out phytochemical profiling essential oils (EOs) and evaluation their anti-microbial activity. Methods: EOs extracted from Cumminum cyminum, Zingiber officinale, Trachyspermum ammi, Alipnia galanga, Cedrus deodara, Elettaria cardamomum using clavenger apparatus. Phytochemical analysis high-performance thin layer chromatography (HPTLC) fingerpri...

As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormone-like adverse effect. Herein, high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification confirmation of proposed validated in vinegar coconut juice. First, detectability to analyte on different layer materials estimated,...