Loxopterygium huasango Spruce ex Engl., of the Anacardiaceae family, classified critically endangered, is a forest tree with considerable value in Seasonally Dry Tropical Forest (SDTF) northwest Peru and south Ecuador. The aim study was callus induction, propagation germplasm conservation using vitro tissue culture techniques. In seed germination (90%) obtained on Murashige Skoog (MS) medium su...

BACKGROUND As a valuable medicinal plant, Madagascar periwinkle (Catharanthus roseus) produces many terpenoid indole alkaloids (TIAs), such as vindoline, ajamlicine, serpentine, catharanthine, vinblastine and vincristine et al. Some of them are important components of drugs treating cancer and hypertension. However, the yields of these TIAs are low in wild-type plants, and the total chemical sy...

Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species arid desert vegetation in China, and it also an ideal material for studying plant stress resistance, which plays extremely role protecting ecosystems preventing land desertification. However, there are relatively few studies on regeneration fusiform, genetic transformation system has not been successfully reported, restricts ...

The tomato (Solanum lycopersicum L.) is a major crop of global economic significance. characterization genes associated with agriculturally important traits often performed using genetic transformation. To achieve an efficient transformation protocol, three components are required, namely, regenerable target tissue, DNA delivery method, and robust transformant selection system. present study wa...

A plant regeneration protocol was devised for Cardiospermum halicacabum by means of aseptically extracted 7 days old hypocotyls forming adventitious shoots on Murashige and Skoog (MS) medium harmonized with 0.7 μM thidiazuron (TDZ) producing a maximum of 18.20 ± 0.98 number of shoots in 94% cultures following 4 weeks. Subsequent subculturing for five passages, on a medium without plant growth r...

Withania somnifera (L) Dunal. and Solanum surattense Burm f., the two medicinally important members of family Solanaceae, were investigated for induction of hairy roots using soil borne bacterium, Agrobacterium rhizogenes. Explants like stem segments, hypocotyls and leaves with midrib were infected with the bacterium in vitro. In both plants, extensive hairy roots were induced from leaf explant...

This research was undertaken for optimizing simple and reproducible protocol on indirect regeneration in tomato using kinetin. The effect of kinetin for efficient callus production was studied and its results were interpreted. Hypocotyls and cotyledons were used as explants and effect on callus derived regeneration was greatly influenced by addition of kinetin in media. The individually positio...

A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...

An efficient method to transform five cultivars of tomato (Lycopersicon esculentum), Micro-Tom, Red Cherry, Rubion, Piedmont, and E6203 is reported. A comparison was made of leaf, cotyledon, and hypocotyl explants on 7 different regeneration media without Agrobacterium tumefaciens cocultivation and on 11 different media with cocultivation. Although all cultivars and explants formed callus and r...