Previous studies with the aid of deuterium as a tracer have shown that alcohol dehydrogenase (1)) lactic dehydrogenase (2)) malic dehydrogenase (3)) and a /?-hydroxysteroid dehydrogenase (4) all catalyze the direct transfer of hydrogen between their respective substrates and the para position of the nicotinamide ring of DPN.’ This hydrogen transfer was also shown to be stereospecific for the DP...

A method is described for the culture of rat ovarian surface epithelial cells, i.e., the cellular component thought to be the source of most ovarian cancers. These cells in culture have a characteristic epithelial morphology which distinguishes them from other ovarian cell types. Cultured surface epithelial cells are histochemically positive for 17ß-hydroxysteroid dehydrogenase and negative fo...

OBJECTIVE Ketoconazole (KCZ) is an anti-fungal agent extensively used for clinical applications related to its inhibitory effects on adrenal and testicular steroidogenesis. Much less information is available on the effects of KCZ on synthesis of steroid hormones in the ovary. The present study aimed to characterize the in situ effects of KCZ on steroidogenic enzymes in primary rat ovary cells. ...

A method is described for the culture of rat ovarian surface epithelial cells, i.e., the cellular component thought to be the source of most ovarian cancers. These cells in culture have a characteristic epithelial morphology which distinguishes them from other ovarian cell types. Cultured surface epithelial cells are histochemically positive for 17 beta-hydroxysteroid dehydrogenase and negative...

Rabbit preimplantation embryos were flushed from the reproductive tract at 24 hr (1- to 2-cell stage), 48 hr (morula), 72 hr (morula), 96 hr (blastocyst), 120 hr (blastocyst), and 144 hr (blastocyst) post coitum. At 168 hr (early postimplantation period), gestation sacs were excised, frozen, and sectioned in a cryostat. Delta5-3beta-Hydroxysteroid dehydrogenase [3(or 17)beta-hydroxysteroid:NAD(...

background the hepatitis b virus x (hbx) protein has long been recognized as an important transcriptional transactivator of several genes. human aldo-keto reductase family 1, member c1 (akr1c1), a member of the family of akr1cs, is significantly increased in hbx-expressed cells. objectives this study aimed to investigate the possible mechanism of hbx in regulating akr1c1 expression in hepg2.2.1...

To assess comparative 3fi-hydroxysteroid dehydrogenase-isomerase and I7fisteroid dehydrogenase activities in human fetal testis and adrenal, equal weights of adrenal and testicular tissue from the same fetuses were incubated with dehydroepiandrosterone or pregnenolone, under identical conditions. Tissues from five fetuses were investigated. The following products were isolated and quanti tated:...

12-Keto-UDCA is an important optically active component for the drug ursodeoxycholic acid (UDCA). Starting from the three-keto compound dehydrocholic acid, the carbonyl groups at position 3 and 7 have to be reduced stereoand regioselectively. In this case we applied two hydroxysteroid dehydrogenases for this purpose, the NAD-dependent 3α-HSDH from Pseudomonas testosteroni and the NADP-dependent...