An experimental in vivo model for studying cartilage destruction has been used to study the possible chondroprotective effect of the unsaponifiable constituents of avocado, soya and their combination at a ratio of 1:2. The method consists of implanting rat articular cartilage wrapped in cotton subcutaneously in mice, treating the animals daily for 2 weeks with the preparations in question, then...

BACKGROUND Failure of anastomotic healing is one of the major complications in colorectal surgery. Because histamine plays an important role in immune and inflammatory reactions, we demonstrate the effects of famotidine on the healing of colonic anastomosis in rats. METHODS Twenty-eight Sprague-Dawley rats were used in the study. Excision and end-to-end anastomosis was performed in the distal...

I . Hydroxyproline was determined in urine, carcass, and skin of young rats fed on lowprotein and low-calorie diets. 2. Protein and calorie deprivation in young rats resulted in a marked fall in the soluble as well as insoluble collagen content of the skin. 3. The collagen content of the carcass (excluding skin) was slightly greater in the deficient rats than in the weanling rats, but very much...

Incubation of cartilage from chick embryos with l*C3,5-cis-hydroxy-DL-proline demonstrated that cis-hydroxyproline is incorporated into the collagen and other protein synthesized by the tissue. Gel filtration of extracts from the tissue demonstrated that the proteins synthesized in the presence of cis-hydroxyproline were about the same size as proteins synthesized under control conditions. Incu...

AIM The purpose of this study was to develop an improved method for collagen and protein assessment of fibrotic lungs while decreasing animal use. METHODS 8-10 week old, male C57BL/6 mice were given a single intratracheal instillation of crocidolite asbestos or control titanium dioxide. Lungs were collected on day 14 and dried as whole lung, or homogenized in CHAPS buffer, for hydroxyproline ...

Background & Objectives: Hepatic fibrosis is the extreme extracellular matrix buildup of collagen due to chronic liver disorders. Progressive results in cirrhosis and hepatic failure. Nilotinib considered a 2nd generation tyrosine kinase inhibitor, which exhibited antifibrotic efficacy. Stem cells release cytokines growth factors paracrine-mediated anti-inflammatory effects vitro vivo. Hence, s...

The radial immunodiffusion assay overestimates the C1q in serum. Here we describe a convenient, accurate procedure for measuring C1q in 250 microL of dialyzed serum. This method is based on our previous findings that all C1q in serum precipitates with the euglobulin fraction and that all other serum proteins containing hydroxyproline are excluded from this fraction. Because C1q is 4.3% hydroxyp...

The effect of different intakes of salt for 12 mo on bone calcium content and urinary excretion of calcium and hydroxyproline were examined in sham operated and oophorectomized (OX) rats to determine the long term effects of high sodium intake and its interaction with estrogen deficiency. Sham operated (n = 24) and OX (n = 24) rats were divided into groups of six rats in a 2 x 4 design. One gro...