Fas-mediated apoptosis has been suggested to contribute to tubular cell death after renal ischemia-reperfusion injury. Here we investigate whether small interfering RNA (siRNA) duplexes targeting Fas protect mice from acute renal failure after clamping of the renal artery. Renal ischemia-reperfusion injury was induced by clamping the renal vein and artery for 15 or 35 min. Mice were treated bef...

We present a microfluidic sample introduction approach based on a novel flow control mechanism, hydrodynamic gated injection. It has the advantages of easy and flexible flow control, similar to its analog, electrokinetic gated injection, but exhibits the unique properties of strong driving force and good biocompatibility, ideal for applications involving live biological samples. Theories for hy...

The electrokinetically pinched method is the most commonly used mode for sample injection in microchip capillary electrophoresis (microCE) due to its simplicity and well-defined sample volume. However, the limited injection volume and the electrophoretic bias of the pinched injection may limit its universal usage to specific applications. Several hydrodynamic injection methods in microCE have b...

This paper shows the applicability of a disposable and inexpensive microfluidic chip for electrochromatographic separations. The chip, recently developed by us for chip-based LC, was fabricated from PDMS incorporating conventional chromatographic RP silica particles (C18) without the use of frits. Three cephalosporin antibiotics were used to demonstrate the applicability of the chip-based chrom...

Stopless flow operation of asymmetrical flow field-flow fractionation (FFF) has been achieved by introducing a hydrodynamic relaxation method using a frit inlet. By using frit inlet injection, a focusing process which has been an essential part of runs at the asymmetrical flow FFF system can be completely avoided. Band broadening of an initial sample zone during hydrodynamic relaxation is discu...

OBJECTIVE von Willebrand Factor (vWF) structure-function relationship has been studied only in vitro. To investigate the physiological importance of particular vWF domains, we have introduced mutations into murine vWF (mvWF) cDNA inhibiting vWF binding to glycoprotein (Gp) Ib, GpIIbIIIa, and to fibrillar collagen. METHODS AND RESULTS We delivered wild-type (WT) or mutant mvWF cDNA into vWF-de...

Short-term expression of transgenes is one of the problems frequently associated with non-viral in vivo gene transfer. To obtain experimental evidence for the design of sustainable transgene expression systems, the contribution of epigenetic modifications to the decline in transgene expression needs to be investigated. Bisulfite sequencing and reactivation by hydrodynamic injection of isotonic ...