Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst...

In the present study, sheep crude hydatid cyst fluid antigen (SCHCFA) was prepared by ammonium sulfate precipitation, centrifugation and dialysis. SDS-PAGE of SCHCFA showed seven polypeptides of 72.8 kDa, 66.5 kDa, 60.2 kDa, 41 kDa, 25.1 kDa, 19 kDa and 8 kDa when stained with Coomassie briliant Blue R-250. The initial part of the ascending loop of the first peak (PI) when resolved by gel filtr...

Complement fixation (CF) is a commonly used serodiagnostic test for hydatid disease, especially recommended for the evaluation of cure after surgery or for checking on treatment with antihelminthic drugs (9). However, the absence of an FDA-approved standardized antigen makes it difficult to assess the performance of different CF tests by using published studies. The objectives of this study wer...

Background: Cystic echinococcosis (CE) is a helminthic disease caused by the larval form of Echinococcus granulosus.  In the present study, the B8/2 subunit of antigen B (AgB) of E. granulosus was expressed in E. coli host and then applied in a diagnostic ELISA set up. Methods: The DNA sequence of AgB8/2 subunit from E. granulosus was extracted from the GenBank and codon-optimized. The target s...

spleen is an unusual location for hydatid cyst. here we report a case of primary splenic hydatid cyst in a 41-yr-old iranian woman from yasuj, southwest of iran. the patient had been admitted to shahid beheshti hospital because of abdominal pain. abdominal sonography revealed a hypoechoic lesion of 150 x 130 mm in the spleen, suggestive of hydatid cyst. splenectomy was performed for the patient...

background: hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of echinococcus granulosus. diagnosis of hydatidosis is still an unresolved problem. serological tests using crude antigens for diagnosis of e. granulosus are sensi-tive, however their specificity are not satisfactory. therefore, who recommended spe-cific serological methods using specific antigens,...

Sera from 71 patients with surgically confirmed hydatid disease (which is caused by Echinococcus granulosus) were studied by an enzyme-linked immunoelectrotransfer blot (EITB) assay. Sera from patients either with other cestode infections or with another illness were used as controls. Results of the EITB test for hydatidosis were compared with those of the double-diffusion (DD5) test and an enz...

1197 The mechanism for the air in the mediastinum is suggested as being due to rupture of marginal pulmonary alveoli, allowing the development of pulmonary interstitial emphysema. This then tracks through the fascial planes at the lung hilum, and from there into the mediasti-num and neck. There was no obvious underlying lung disease in our patient, and no further investigations were indicated. ...

A study was undertaken to assess the sensitivity and specificity of Counter Immunoelectrophoresis (CIE) in the diagnosis of hydatidosis in buffaloes using sera samples collected at the time of slaughter. One hundred sera samples from buffaloes were screened for hydatidosis using fertile buffalo hydatid cyst fluid as antigen by CIE. Out of the 100 sera samples used, 61 were positive for hydatid ...