background detection of fastidious enteropathogenic bacteria in fecal samples of patients with gastroenteritis is a challenge in clinical microbiological laboratories. objectives the aim of this study was to compare the detection limits of the pcr and culture methods for the diagnosis of campylobacter spp., yersinia spp., clostridium perfringens, and clostridium difficile in human stool samples...

Denaturing High-Performance Liquid Chromatography (DHPLC) is a recently developed technique forthe detection of single nucleotide polymorphisms (SNPs) and mutations. It involves the comparisonbetween two or more DNAs as a mixture of denatured and reannealed PCR products. The methodologyis based on the principle of reversed phase liquid chromatography and uses a unique DNA sepa...

The y-glutamyl transferase isoenzymes of bile were studied using electrophoretic, gel filtration, and ultracentrifugation techniques. In view of the known association of other biliary enzymes with lipids the effects of butanol extraction were investigated. The results show the presence of four isoenzymes ofy-glutamyl transferase in bile, differing in electrophoretic mobilities, molecular size, ...

The paternally inherited Y chromosome has been widely used in population genetic studies to understand relationships among human populations. Our interpretation of Y chromosomal evidence about population history and genetics has rested on the assumption that all the Y chromosomal markers in the male-specific region (MSY) are selectively neutral. However, the very low diversity of Y chromosome h...

We examined the biogeographic patterns implied by early hominid phylogenies and compared them to the known dispersal patterns of Plio-Pleistocene African mammals. All recent published phylogenies require between four and seven hominid dispersal events between southern Africa, eastern Africa, and the Malawi Rift, a greater number of dispersals than has previously been supposed. Most hominid spec...

The Y-chromosome is responsible for sex determination in mammals, which is triggered by the expression of the SRY gene, a testis-determining factor. This particular gene, as well as other genes related to male fertility, are located in the non-recombining portion of the Y (NRY), a specific region that encompasses 95% of the human Y-chromosome. The other 5% is composed of the pseudo-autosomal re...

1. The analysis, by silica-gel chromatography, of phospholipids from a human-plasma fraction is described. The fraction was derived from whole blood which had been incubated with sodium [Me-14C]acetate for 6-5 hr. The #-lipoproteins had been removed by precipitation with dextran sulphate. 2. All the phospholipids isolated were labelled. 3. The fatty acids of the isolated phospholipids were anal...