Objective(s):Transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. Melatonin is secreted from the pineal gland and some organs such as testes. According to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantatio...

Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. MaterialsAndMethods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (...

objective: we evaluated structural and functional changes of fresh and frozen-thawed adult mouse spermatogonial stem cells following auto-transplantation into gamma-irradiated testes. materials and methods: in this experimental research, the right testes from adult mice (n=25) were collected, then sertoli and spermatogonial cells were isolated using two-step enzymatic digestion, lectin immobili...

BACKGROUND Spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (SSCs) in testis tissue and during the culture; therefore we undertook this study ...

Introduction: Nowadays, spermatogonial stem cells (SSCs) cultivation has been used by many researchers as an effective tool for infertility treatments. Oxidative conditions can be effective on cell proliferation and differentiation of these cells. So, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during SSCs in vitro culture. ...

background: the present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. materials and methods: sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. isolated spermatogonial cells were co-cultured with sertoli cells in minimal essential medi...

Stem cells have a potential of gene therapy for regenerative medicine. Among various stem cells, spermatogonial stem cells have a unique characteristic in which neighboring cells can be connected by intercellular bridges. However, the roles of intercellular bridges for stem cell self-renewal, differentiation, and proliferation remain to be elucidated. Here, we show not only the characteristics ...

Recently new reports have proved the pluripotency of spermatogonial stem cells (SSCs) derived from male gonad. This pluripotent stem cells resembled Embryonic stem cells recognized as Embryonic Stem like cells (ES like cells). ES like cells forms sharp edge colonies that are immunopositive to pluripotency markers and have differentiation capacity to Ectodermal, Mesodermal and Endodermal layers....

Although stem cells are believed to divide infinitely by self-renewal division, there is little evidence that demonstrates their infinite replicative potential. Spermatogonial stem cells are the founder cell population for spermatogenesis. Recently, in vitro culture of spermatogonial stem cells was described. Spermatogonial stem cells can be expanded in vitro in the presence of glial cell line-...