AIM To study the influence of enterococci on human sperm membrane in vitro. METHODS Ejaculated human sperm were artificially infected with beta-hemolytic or non-beta-hemolytic enterococci at the bacteria: sperm ratio of 50:1 at 37 degrees . Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling (HOS) test and electron microscopy. RESULTS Sperm infect...

Among the numerous anti-sperm monoclonal antibodies generated in our laboratory, HS-33 was shown to react with a conserved antigen on the acrosome of spermatozoa from human and mouse. By using indirect immunofluorescent assay, it was demonstrated that HS-33 did not bind to live human sperm. However, this antibody was found to react with the methanol-fixed acrosome-intact, but not with acrosome-...

new advances in mass spectrometry-based proteomics technology are having amajor impact on our understanding of how human spermatozoa acquire their capacity for fertilization. a complete analysis of the proteins found in the human spermatozoa is essential for understanding the events leading up to, and including, fertilization and early embryo development. in this short review, we have collected...

Spermatozoa generate reactive oxygen species (ROS) in physiological amounts which play a role in sperm functions during sperm capacitation acrosome reaction (AR) and oocyte fusion. In addition damaged sperm are likely to be the source of ROS. The most important ROS produced by human sperm are hydrogen peroxide superoxide anion and hydroxyl radicals. Besides human seminal plasma and sperm posses...

BACKGROUND Curcumin has shown to affect sperm motility and function in vitro and fertility in vivo. The molecular mechanism(s) by which curcumin affects sperm motility has not been delineated. Since modulation of intracellular pH (pHi) and plasma membrane polarization is involved in sperm motility, the present study was conducted to investigate the effect of curcumin on these sperm (human and m...

Introduction: The novel development of the (HBA) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. The purpose of this study was to evaluate the correlation between HBA score and histone deficiency, protamine content and DNA fragmentation. Methods: During April- March 2012 patients who were enrolled at ...

Background: Nowadays, cryoprotectant-free vitrification of human sperm in large or small volumes facilitated storage and use of human sperm for long time. However, this procedure was not successful in farm animals. The main finding in the studies is higher sensitivity of these kinds of sperm to sucrose osmolarities. Preliminary studies showed a reversible effect of sucrose on goat epididymal sp...

Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...

BACKGROUND The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro. METHODS Sperm samples from 129 infertile men were studied. Motile sperm (2 x 10(6)) selected by Pure sperm were incubated with four oocytes in 1 ml human tubal fluid supplemented wit...