Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleli...

dried aceton-chloroform extract of aerial parts of euphorbia spinidens bornm. ex prokh. endemic to iran, yielded two new triterpenoids, lup-20(29)-ene-33, 28 diol commonly known as betulin and (3β,23e)-cycloarta-23-ene-3,25-diol. the structures of the isolated compounds were elucidated by 13 c- and 1 h-nmr as well as 2d-nmr, ir and by the aid of mass fragmentation pattern. in phagocyte chemilum...

Lymphocyte recirculation begins with the attachment of circulating cells to the structurally distinctive postcapillary venules of lymphoid organs termed high-endothelial venules (HEVs). In both rodents and humans, the attachment of lymphocytes to the HEVs of peripheral lymph nodes (PNs) on the one hand and gut-associated lymphoid tissues (GALTs) on the other appears to involve discrete adhesive...

background: salvia mirzayanii, a native plant to iran, is shown to have immunomodulatory effects on lymphocyte proliferation. objective: to identify the bioactive immunomodulatory compound(s) present in s. mirzayanii. methods: the crude extract was fractionated to five fractions in two steps using different solvents. the fractions were subjected to bioassay-guided fractionation. all the fractio...

Preincubation of human blood lymphocytes with cell surface antigen specific antibodies under non-capping conditions reduces the electrophoretic mobility of the corresponding lymphocyte subpopulation. Antigen-positive and antigen-negative cells can be separated by free flow electrophoresis with high yield, purity and viability. The use of fluorescence-labelled second antibodies augments the indu...

The production of interferon (IFN) was investigated in human peripheral lymphocyte cultures stimulated by mumps virus. IFN-alpha and IFN-gamma were produced in lymphocyte cultures from immune donors. However, no IFN-gamma was produced in lymphocyte cultures from nonimmune donors. IFN-alpha was produced by B lymphocytes, and IFN-gamma was produced by T lymphocytes. An increase in the production ...

Lymphocyte trafficking is a fundamental aspect of the immune system that allows B and T lymphocytes with diverse antigen recognition specificities to be exposed to various antigenic stimuli in spatially distinct regions of an organism. A lymphocyte adhesion molecule that is involved with this trafficking phenomenon has been termed the homing receptor. Previous work (Lasky, L., T. Yednock, M. Si...