A simple, rapid, precise and accurate HPLC – UV procedure has been developed to determine benzaldehyde, the toxic oxidation product of the widely used preservative and Co – solvent, benzyl alcohol, in injectable formulations. Benzaldehyde as a main potential impurity of benzyl alcohol may cause many toxic effects such as neurotoxicity, hypersensitivity in human and fatal toxic syndrome in prema...

The popularity of imatinib in modern medicine has underscored the need for a fast and effective quantification method without the use of expensive instruments. The aim of this study was to develop and validate a relatively fast, cheap and effective HPLC method for the quantification of imatinib in human serum. This method uses a simple preparation step with an Extrelut® NT 3 extraction tube and...

Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV d...

two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of sulfamethoxazole (smx) and phthalazine (phz) by high performance liquid chromatography (hplc). multivariate calibration techniques such as classical least squares (cls) and inverse least squares (ils) were introduced into hplc to determine the quantification by using uv detecto...

An HPLC method with UV detector was developed for the determination of DEHP phthalate ester in alcoholic beverage “Ouzo”. Phthalate esters are added to plastic packaging food and beverages increase flexibility, transparency, strength, longevity. When these substances come into contact or beverages, they can lead migration residues product. This paper presents a two-step process involving extrac...

Measurement of 17-hydroxyprogesterone (17-OHP) in daily dried blood spot profiles via radioimmunoassay is a convenient and accepted method for monitoring of glucocorticoid therapy in Congenital Adrenal Hyperplasia. Characteristics of this method that serve to limit its clinical usefulness include its lack of specificity for 17-OHP, and a relatively high limit of detection. Mass Spectroscopy is ...

Crocins in commercial liquid saffron extracts (Saffr’activ®) were identified using high-performance chromatography (HPLC) with a diode array detector (DAD) and mass spectrometry (MS). The impact of storage on the qualities extract studied HPLC-DAD-MS by exposing trans-4-GG crocin to environmental factors. Light temperature induced degradation after only one week. Trans-4-GG was totally hydrolyz...

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based qua...

OBJECTIVES The aim of this work was to develop and validate an HPLC method for gentamicin quantification in different types of biological samples such as animal tissues and cellular material and also in pharmaceuticals. METHODS Poly(lactide-co-glycolide) microparticles (MP) of gentamicin (PLGA 502H MP), THP-1 cells, and plasma and tissue samples of mice treated with the antibiotic either free...