Abbreviations : apo, apolipoprotein; CE-OOH, cholesteryl ester hydroperoxides; RP-HPLC, reversed-phase high-performance liquid chromatography; LDL, low-density lipoprotein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TBARS, 2-thiobarbituric acid-reactive substances; TBS, Tris-HCl-buŠered saline; a-Toc, a-tocopherol; VLDL, very-low-density lipoprotein Biosci. Biotechnol....

Complex I purified from bovine heart mitochondria is a multisubunit membrane-bound assembly. In the past, seven of its subunits were shown to be products of the mitochondrial genome, and 35 nuclear encoded subunits were identified. The complex is L-shaped with one arm in the plane of the membrane and the other lying orthogonal to it in the mitochondrial matrix. With mildly chaotropic detergents...

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

Cereal Chern. 65(3):215-222 Zein, the major alcohol-soluble protein of corn endosperm, was extracted with 55% 2-propanol. It was then fractionated by preparative reversed-phase (CIs) high-performance liquid chromatography (RPH PLC) with an acetonitrile-triOuoroacetic acid gradient. From 15 to 17 peaks resulted for native or reduced and alkylated (R-A) zeins. Sodium dodecyl sulfate-polyacrylamid...

objective: with consideration of lethal effects of aflatoxins specially b1 on human health. estimation of aflatoxin-albumin adduct, as an important marker of aflatoxin exposure, seems essential. the aim of this study is optimization of hplc-fluorescence method for measurement of this important marker in blood serum. materials and methods: in this study, blood serum of three groups of rats as a...

the 1rs chromosome segment derived from petkus rye carries genes for resistance to three wheat rust diseases, namely lr26 for resistance to leaf rust (caused by puccinia triticina), yr9 for resistance to stripe rust (p.striiformis f. sp. tritici) and sr31 for resistance to stem rust (p. graminis f. sp. tritici). since sec-1 is tightly linked with the three rust resis-tance genes electrophoresis...

the purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). i...