Transferrin receptor 2 (TfR2) is a homolog of transferrin receptor 1 (TfR1), the receptor responsible for the uptake of iron-loaded transferrin (holo-Tf) into cells. Unlike the ubiquitous TfR1, TfR2 is predominantly expressed in the liver. Mutations in TfR2 gene cause a rare autosomal recessive form of the iron overload disease, hereditary hemochromatosis. Previous studies demonstrated that hol...

This work presents the Apo-Holo DataBase (AH-DB, http://ahdb.ee.ncku.edu.tw/ and http://ahdb.csbb.ntu.edu.tw/), which provides corresponding pairs of protein structures before and after binding. Conformational transitions are commonly observed in various protein interactions that are involved in important biological functions. For example, copper-zinc superoxide dismutase (SOD1), which destroys...

Im Rahmen seiner Markteinfuhrung wurde ein neuartiges Lebensmittel fur besondere medizinische Zwecke von einem multidisziplinaren Expertengremium als Option Allergiepatienten diskutiert. Die neu entwickelte Lutschtablette enthalt neben dem Molkeprotein β-Lactoglobulin (BLG) auch die Mikronahrstoffe Eisen, Zink und Vitamin A. Das mit Liganden beladene BLG (holo-BLG) wird Faktor des allergieprote...

An antitumor antibiotic C-1027, a complex protein consisting of an apoprotein and a non-covalently bound chromophore, showed some aminopeptidase activity, 1/15 (on the basis of activity per mg protein) that of porcine kidney enzyme [E.C. 3.4.11.2] by use of L-phenylalanyl 4-methyl-coumaryl-7-amide as the substrate. Neither the apoprotein alone nor the chromophore alone were active. Amastatin an...

BACKGROUND We examined the effect of oral vitamin B(12) treatment on fluctuations in plasma total cobalamin and its binding proteins transcobalamin (TC) and haptocorrin (HC). METHODS Patients (n = 88; age range, 38-80 years) undergoing coronary angiography (part of the homocysteine-lowering Western Norway B-Vitamin Intervention Trial) were allocated to daily oral treatment with (a) vitamin B(...

Heme-linked proteins, such as cytochromes, are popular subjects for protein folding studies. There is the underlying question of whether the heme affects the structure of the denatured state by cross-linking it and forming other interactions, which would perturb the folding pathway. We have studied wild-type and mutant cytochrome b562 from Escherichia coli, a 106 residue four-alpha-helical bund...

association between the 259R allele and lower concentrations of holo-TC in plasma (8, 21). Nevertheless, holo-TC in CSF was reduced in patients with the 259PR and 259RR genotypes, showing that the polymorphism indeed affected holo-TC concentrations in CNS. Holo-TC concentrations in plasma and CSF were highly correlated, which is compatible with the notion that all holo-TC in CSF originates from...

Holographic television, or “holo-video,” has been seen by many as the ultimate development not only of holography but of electronic visual communication generally. To make widespread, successful holo-video, four things are needed: 1) content, 2) a distribution mechanism, 3) sufficient processing at the receiver, and 4) suitable electro-optics at the display, and all of these must be available a...

Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for produ...

TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood. The urea-induced reversible denaturations of the apo- and holo-enzy...