Microbes for environmental research should be cultured in growth media with characteristics (e.g., pH, ionic strength, and ionic composition) as close to their original habitat as possible. Additionally, the medium should also enable high cell density to be obtained, which is needed for providing sufficient cells in subsequent experiments. This in-progress report describes the formulation of a ...

A marine photosynthetic bacterium (PS88) identified as Rhodovulum sp. with flocculating ability was isolated from the sea sediment mud of a shrimp cultivation farm in Thailand. This bacterium flocculated in glutamate/malate medium during aerobic/dark or anaerobic/light cultivation. The flocculating ability was enhanced with the increase of NaCl concentration to 6% (w/v). Flocculation and growth...

This work aims at optimizing the high cell density fungal cultivation for producing large quantities of fungal biomass to be used in azo dye residues bioremediation. In our previous studies the efficacy of using certain fungal strains to decolorize a range of commercial textile dyes of different structures (azo, disazo) were investigated. Several promising fungal strains belonging to Aspergillu...

For improved interfacing of the Pichia pastoris fed-batch cultivation process with expanded bed adsorption (EBA) technique, a modified cultivation technique was developed. The modification included the reduction of the medium salt concentration, which was then kept constant by regulating the medium conductivity at low value (about 8 mS/cm) by salt feeding. Before loading, the low conductivity c...

Articular chondrocytes are known to be phenotypically unstable in culture. One condition that has been reported to suppress dedifferentiation is cultivation at high density on tissue-culture plastic. The aim of the experiments described here was to study the effect of seeding density on chondrocyte proliferation and 35SO4 incorporation, and on the types of collagen and proteoglycan synthesized....

Fibroblast cell cultivation method was evaluated by using natural polymers sourced from pulp cellulose in their hydrogel forms. The pulp sourced was offered as an alternative for the preparation of hydrogel films when the cellulose was dissolved in dimethylacetamide/lithium choride (DMAc/LiCl) solution and converted hydrogels having flexible and transparent properties. The cultivation of the fi...