This work aims to automatically detect cement lines in decalcified cortical bone sections stained with H&E. Employed is a methodology developed previously by the authors and proven to successfully count and disambiguate the micro-architectural features (namely Haversian canals, canaliculi, and osteocyte lacunae) present in the secondary osteons/Haversian system (osteon) of cortical bone. This m...

I t is known that a continuous process takes place in bone whereby mature osteones (Haversian systems) are broken down and new osteones are formed. Historadiographic study has shown that the mineral salts content of osteones increases gradually toward a maximum (1, 2, 8). In order to obtain information on the chemical and physical properties of the growing osteone, we have developed a dissectio...

Distinguishing human from non-human bone fragments is usually accomplished by observation of gross morphology. When macroscopic analysis is insufficient, histological approaches can be applied. Microscopic features, like plexiform bone or osteon banding, are characteristic of non-humans. In the absence of such features, distinguishing Haversian bone as either human or non-human proves problemat...

Intra-cortical bone remodelling is a cell-driven process that replaces existing bone tissue with new bone tissue in the bone cortex, leaving behind histological features called secondary osteons. While the scaling of bone dimensions on a macroscopic scale is well known, less is known about how the spatial dimensions of secondary osteons vary in relation to the adult body size of the species. We...

BACKGROUND Exposure to cadmium (Cd) is considered a risk factor for various bone diseases in humans and experimental animals. This study investigated the acute effects of Cd on femoral bone structure of adult male rats after a single intraperitoneal administration. METHODS Ten 4-month-old male Wistar rats were injected intraperitoneally with a single dose of 2 mg CdCl2/kg body weight and kill...

Whereas recent work has demonstrated the role of oxygen tension in the regulation of skeletal cell function and viability, the microenvironmental oxemic status of bone cells remains unknown. In this study, we have employed the Krogh cylinder model of oxygen diffusion to predict the oxygen distribution profiles in cortical and cancellous bone. Under the assumption of saturation-type Michaelis-Me...

In this study, the fracture micromechanics of Haversian cortical bone has been considered. To this effect, a two-dimensional micromechanical fibre-ceramic matrix composite tissue materials model has been presented. The interstitial tissue was modeled as a matrix and the osteon was modeled as a fibre, followed by the application of linear elastic fracture mechanics theory. The solution for edge ...