Thielaviopsis basicola and Tomato spotted wilt virus (TSWV) are the most important problems in a moderate climate zone. Previously obtained doubled haploids (DH) of F1 hybrids of the flue-cured line WGL3 resistant to Th. basicola and the dark-cured line PW-834 carrying RTSW-al gene provided the research material. Biological tests and SCAR markers linked with TSWV were applied to confirm resista...

The frequency of haploid production following the inlerspecific pollination of eight tetraploid pot<lto cuhi var~ (Solanum wherosum subsp. IIlberosum) with Solanum phureja clone 1.22 was investigated. A total of 185 haploids were produced with an overall haploid frequency of 3.9 haploids/lOO [ruits. The haploid frequency was affected by the genotype of the maternal parent. Atlantic, NDR60-2, Su...

Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific induction cereals. Although vivo system based on OsMTL-edited plants has been established rice (Oryza sativa), DH still limited by other factors, such as identification, which one of essential steps required ...

This pioneering work reports successful androgenic plant development via embryogenesis from microspore calluses in anther cultures and estimation of bioactive metabolites vitro regenerants parent (control) Cambod tea, Camellia assamica ssp. lasiocalyx (Planch MS) cultivar TV19. Anthers bearing microspores at early-to-late uni-nucleate stage were selected to initiate androgenesis. A pre-treatmen...

A. Pratap and J. Kumar (eds.), Alien Gene Transfer in Crop Plants, Volume 1: Innovations, Methods and Risk Assessment, DOI 10.1007/978-1-4614-8585-8_2, © Springer Science+Business Media New York 2014 Abstract Wide or distant hybridization has been widely used as an important tool of chromosome manipulation for crop improvement. The chromosome behaviors in F 1 hybrids provide us with the essenti...

Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germin...

De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...

Ploidy is predicted to influence adaptation directly, yet whether single mutations behave the same in different ploidy backgrounds has not been well studied. It has often been assumed theoretically that aside from dominance, selective parameters do not differ between cells of varying ploidy. Using the budding yeast Saccharomyces cerevisiae, I compared the effect size of 20 adaptive mutations in...

Cassava breeding is difficult and, compared with other crops, inefficient. The problems in cassava breeding relate to the length of the breeding cycle, the large genetic load present in the crop, and the heterozygous nature of the parents and progenies evaluated. The production of doubled haploids, through tissue culture techniques, offers interesting advantages. By definition, any process that...