Wu, Feng, Frank Park, Allen W. Cowley, Jr., and David L. Mattson. Quantification of nitric oxide synthase activity in microdissected segments of the rat kidney. Am. J. Physiol. 276 (Renal Physiol. 45): F874–F881, 1999.—This study was designed to quantify nitric oxide synthase (NOS) activity in microdissected glomeruli (Glm), pars convoluta, pars recta, cortical collecting duct, cortical thick a...

Coffin, Benoit, Sophie Fossati, Bernard Flourié, Marc Lémann, Pauline Jouet, Claire Franchisseur, Raymond Jian, and Jean-Claude Rambaud. Regional effects of cholecystokinin octapeptide on colonic phasic and tonic motility in healthy humans. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G767–G772, 1999.—The aim of this study was to assess in nine healthy subjects the effects of CCK octa...

We compared the pathogenicity of intimin-negative non-O157:H7 Shiga toxin (Stx)-producing Escherichia coli (STEC) O91:H21 and O104:H21 strains with the pathogenicity of intimin-positive O157:H7 and O157:H(-) strains in neonatal pigs. We also examined the role of Stx2d-activatable genes and the large hemolysin-encoding plasmid of O91:H21 strain B2F1 in the pathogenesis of STEC disease in pigs. W...

Unveiling the genetic architecture of grain yield and yield-related traits is useful for guiding the genetic improvement of crop plants. Kernel row number (KRN) per ear is an important yield component, which directly affects the grain yield of maize. In this study, we constructed a set of 130 chromosome segment substitution lines (CSSLs), using Nongxi531 as the donor parent and H21 as recipient...

In recent years, the number of wheat, Triticum aestivum L., fields heavily infested by Hessian fly, Mayetiola destructor (Say), has increased in the Great Plains of the United States. Historically, resistance genes in wheat have been the most efficient means of controlling this insect pest. To determine which resistance genes are still effective in this area, virulence of six Hessian fly popula...

Ortmann, Sylvia, and Gerhard Heldmaier. Regulation of body temperature and energy requirements of hibernating Alpine marmots (Marmota marmota). Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278: R698–R704, 2000.— Body temperature and metabolic rate were recorded continuously in two groups of marmots either exposed to seasonally decreasing ambient temperature (15 to 0°C) over the entire ...

The effects of ambient pH on postmoult net fluxes of Ca, acidic/basic equivalents (H+, NH4+/OH2, HCO3), Na and Cl2, total body Ca, haemolymph pH and electrolyte status were assessed in the freshwater crayfish Procambarus clarkii (Girard). Variables were monitored for 5 days postmoult in acidic (pH5.2; H2SO4) or alkaline (pH9.2; KOH) artificial tap water (ATW) and compared with those in control ...

background: the purpose of this study was to find out the frequency of different serotypes of enteropathogenic es­cherichia coli (epec) among healthy/diarrheal cases. methods: a total of 191 strains, 111 from diarrheal and 80 from asymptomatic persons were examined. determination of the epec sero­groups was performed by agglutination tests using polyvalent and monovalent o antiserum. pcr-rflp a...

Shiga toxin-producing Escherichia coli O157:H7 (STEC) is by far the most prevalent serotype associated with hemolytic uremic syndrome (HUS) although many non-O157 STEC strains have been also isolated from patients with HUS. The main virulence factor of STEC is the Shiga toxin type 2 (Stx2) present in O157 and non-O157 strains. Recently, another toxin, named subtilase cytotoxin (SubAB), has been...

Authors M.E. Pintado, A.I.E. Pintado, and F.X. Malcata are affiliated with Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200 Porto, Portugal. Address inquiries to Dr. F.X. Malcata. ABSTRACT The rates were monitored on biomass increase, polysaccharide production and viscosity development of whey broth and a control synthetic broth d...