Spheroid cultures of human hepatoblastoma cells (HuH-6 line) were established by rotating 3 x 10(6) cells/3 ml culture medium in 25-ml Erlenmeyer flasks on a gyratory shaker. The size of the spheroids rapidly increased until 4 days of culture, and thereafter their size gradually increased until 8 days of culture. A considerable amount of lactate dehydrogenase (LDH) was detected in the culture m...

Baby hamster kidney fibroblasts (BHK21 cells) transformed by polyoma or Rous sarcoma viruses aggregate less than the untransformed parental cells when incubated in growth medium in a gyratory shaker for 18-24 h. This difference can be measured by electronic particle counting, or by filtering aggregated suspensions of 32P-labelled cells through bolting fabric. The aggregation of transformed deri...

The presence of small numbers of tumour cells inhibits the aggregation of embryonic chicken neural retina cells grown in gyratory shaker culture. The aggregation of neural retina cells was also inhibited by ascites cell medium. We investigated whether the inhibitory effect of the tumour cells on aggregate size is effected by inhibition of the initial adhesion or by enhancement of their separati...

This study evaluates the short-term resilient behaviour of cold in-place recycled (CIR) asphalt mixtures with bitumen emulsion when they are more similar to non-cohesive granular materials than conventional hot mixtures. Gyratory compacted CIR specimens were manufactured using different proportions bituminous and water, dynamic triaxial tests conducted at curing times. The moduli obtained exper...

Skeletal muscle and liver tissue from 0,-day-old chick embryos were dissociated into separate cells using 0-25 % (w/v) crude trypsin. The effect of rabbit anti-actomyosin sera on the aggregation of these cells was estimated by the gyratory shaker and turbidimetric methods. Studies were also undertaken on the ability of fluorescein isothiocyanate-labelled rabbit anti-uterine actomyosin serum (FI...

The Superpave gyratory compactor (SGC) is used to compact asphalt specimens for mixture testing in the Superpave system. Five companies currently manufacture SGCs for use in the United States, offering a total of eight different models. Each model employs a unique method of setting, inducing, and maintaining the specified angle of gyration. Calibration systems are required for each device for t...