Growth plates are specialized cartilage structures that ensure the elongation of most skeletal primordia during vertebrate development. They are made by chondrocytes that proliferate in longitudinal columns and then progress in a staggered manner towards prehypertrophic, hypertrophic and terminal maturation. Complex molecular networks control the formation and activity of growth plates, but rem...

Bones elongate through endochondral ossification in cartilaginous growth plates located at ends of primary long bones. Linear growth ensues from a cascade of biochemical signals initiated by actions of systemic and local regulators on growth plate chondrocytes. Although cellular processes are well defined, there is a fundamental gap in understanding how growth regulators are physically transpor...

Serial dilutions of blood and spleen biopsy specimen, plated on NNN-blood agar using micro-titer culture plates is a sensitive and reproducible method for detection and growth of Leishmania parasites. Plates could easily be monitored and growth rapidly detected. Moreover, parasite number may be estimated using this technique. Key wordsvisceral leishmaniasis, peripheral blood, splenic biopsies, ...

GWAS have identified hundreds of height-associated loci. However, determining causal mechanisms is challenging, especially since height-relevant tissues (e.g. growth plates) are difficult to study. To uncover mechanisms by which height GWAS variants function, we performed epigenetic profiling of murine femoral growth plates. The profiled open chromatin regions recapitulate known chondrocyte and...

Plant growth and development can be influenced by mutualistic and non-mutualistic microorganisms. We investigated the ability of the ericoid endomycorrhizal fungus Oidiodendron maius to influence growth and development of the non-host plant Arabidopsis thaliana. Different experimental setups (non-compartmented and compartmented co-culture plates) were used to investigate the influence of both s...

BACKGROUND This study was carried out to determine growth of dermatophytes using human stratum corneum in vitro and the degrading effect of Keratinases (Proteinases) on stratum corneum for a complete understanding of the host parasite relationship. METHOD Trichophyton rubrum isolates derived from patients with tinea cruris infections were obtained from the Department of Medical Microbiology, ...

The in vitro fungitoxic activity of crude extracts of neem (Azadirachta indica) (A.) Juss) and pawpaw (Carica papaya) (L.) on Alternaria solani, isolated from rotting yam tubers whose pathogenicity has been proven and assessed. The organic solvent (methanol) extracts of leaves of neem and pawpaw at 20%, 40%, 60% and 80% concentrations were tested on potato dextrose agar (PDA) for activity again...

Idiopathic scoliosis is one of the most common disabling pathologies of children and adolescents. Etiology and pathogenesis of idiopathic scoliosis remain unknown. To study the etiology of this disease we identified the cells' phenotypes in the vertebral body growth plates in patients with idiopathic scoliosis. Materials and methods: The cells were isolated from vertebral body growth plates of ...

Introduction: Growth and proliferation of the remaining microorganisms within the root canals may destroy the surrounding tissue of the root and leads to periapical lesion. Consequently, the complete elimination of microorganisms from the root canal is a n important goal of endodontic therapy. Endodontic sealers do not provide complete seal in root canal system, and micro spaces have always rem...

PURPOSE This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. MATERIALS AND METHODS Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP p...