Objective: Myh11 encodes a myosin heavy chain protein that is specifically expressed in smooth muscle cells (SMCs) and important for maintaining vascular wall stability. The goal of this study to generate dual reporter mouse line definitive visualization MYH11 + SMCs vivo. Approach Results: We generated knock-in model by inserting LoxP-nlacZ-4XpolyA-LoxP-H2B-GFP-polyA-FRT-Neo-FRT cassette into ...

background : acanthamoeba castellanii forms a resistant cyst that protects the para-site against the host’s immune response. acanthamoeba type-i metacaspase (acmcp) is a caspase-like protein that has been found to be expressed during the encysta-tions. dictyostelium discoideum is an organism closely related to acanthamoeba useful for studying the molecular function of this protozoan caspase-lik...

This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DC...

Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this stu...

Plant viruses classified in the genus Waikavirus , family Secoviridae are positive sense single-stranded RNA that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical RTSV). Many aspects molecular biology waikaviruses remain unexplored because experimental challenges lack infectious clones for low titer, phloem-limited, obligately vector-trans...

Green fluorescent protein (GFP) is a reporter widely utilized to evaluate gene transfer efficiency and used as control in therapeutic overexpression strategies. Although it frequently considered be an innocuous protein, there also evidence indicating interference with contractile function electrophysiological properties, potentially leading cardiac dilation functional deterioration. Cognizant o...

Photosynthetic Cyanobacteria can be used as a chassis for different synthetic biology approaches. However, quantitative comparison of tools engineering, such those heterologous gene expression, is often not available. Here, we report the comparative quantification protein production in Synechococcussp.PCC 7002 regarding expression cassettes and locations foreign integration using sf-GFP reporte...

Superinfection exclusion (SIE) is an antagonistic interaction between identical or closely related viruses in host cells. Previous studies by us and others led to the hypothesis that SIE was elicited one more proteins encoded genomes of primary viruses. Here, we tested this using Turnip mosaic virus (TuMV), a member genus Potyvirus family Potyviridae, with significant economic consequences. To ...