Coffee has been drunk for millennia due to its taste and health benefits. High levels of polyphenols, especially flavonoids phenolic acids, are found in coffee contribute significantly flavor health-giving properties. In this study the total contents, antioxidant, tyrosinase inhibition green coffee, Turkish traditional extracts were evaluated. Antioxidant activities coffees examined by two diff...

The potency of influenza vaccine is determined based on its hemagglutinin (HA) content. In general, single radial immunodiffusion (SRID) assay has been utilized as the standard method to measure HA content. However, preparation of reagents for SRID such as antigen and antibody takes approximately 2~3 months, which causes delays in the development of influenza vaccine. Therefore, quantification ...

Reversed-phase high-performance liquid chromatography (RP-HPLC) was compared with the classical Steelman-Pohley bioassay (BA), based on animal use, for the determination of human follicle-stimulating hormone (hFSH) biological activity. A linear relationship (BA(IU)=0.9925 RP-HPLC(IU)-1.3165) with a highly significant correlation (r=0.9371; p<0.0001; n=24) was found for these two methods for six...

A combination of hydrophobic chromatography on phenyl-Sepharose and reversed phase HPLC was used to purify individual tRNAs with high specific activity. The efficiency of chromatographic separation was enhanced by biochemical manipulations of the tRNA molecule, such as aminoacylation, formylation of the aminoacyl moiety and enzymatic deacylation. Optimal combinations are presented for three dif...

oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

oligosaccharide prepared from Pl.-B was eluted as a single peak on both reversed-phase {RP-) HPLC and size-fractionation (SF-) HPLC, and its strueture was estimated to be Manoct-qManal -. 3)(Xylfi1-.2) Manfi1-4GlcNAcfi1-4(Fuc=1-3)GlcNAc by a combination of component analysis, successive exogl)'cosidase digestions, IS-MS anal)'sis, and 500MHz 'H-NMR, Two tryptic gl)'copeptides were isolated from...

An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...