A set of full-length cDNA clones of the satellite RNA of grapevine fanleaf nepovirus isolate F13 (GFLV-F13) was constructed with a variable number of additional, non-viral nucleotides at the 5' and 3' ends. The biological activity of the RNAs transcribed from these constructs was tested in Chenopodium quinoa protoplasts using a helper virus. When inoculated with arabis mosaic virus S (ArMV-S) R...

Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic modifications of the host endomembrane system that eventually culminate in the formation of a perinuclear "viral compartment." We identified by immunoconfocal microscopy this compartment as the site of virus replication since it contained t...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

Grapevine Pinot gris virus (GPGV) has been identified in many grape growing countries of the world since 2012. The aim this work was to investigate presence GPGV on some accessions collected from a germplasm collection, addition propagation material and clonal selection samples. During 2019–2020, total 199 samples have analysed by double antibody sandwich – enzyme-linked immunosorbent assay (DA...

fanleaf degeneration, a disease caused by grapevine fanleaf virus (gflv), is considered as the most destructive grapevine viral disease worldwide causing severe losses in vineyards, annually. gflv is apparently believed to have originated from ancient persia and therefore, to restrict it in its further infections, a precise identification along with a monitoring of its distribution pattern is c...

There is increasing interest worldwide in how decision-making processes concerning controversial technological innovations can be improved by better integration of input from society at large. Aiming to gain knowledge on grapevine defence mechanisms against grapevine fanleaf virus (GFLV), a severe disease, scientists wanted to carry out a field trial of genetically modified grapevine rootstocks...

Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins. This passage occurs via several distinct mechanisms one of which is MP-dependent formation of the tubules that traverse PD and provide a conduit for virion trans...

a pair of degenerate primers, gmpf1 and gmpr1, was designed on the basis of alignment of previously reported grapevine fanleaf virus (gflv) movement protein (mp) nucleotide sequences from iran and other parts of the world. cdna was synthesized by the use of oligo d(t)18 from total rna extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (pcr) with the d...

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved wi...