We have investigated the capacity of polymorphonuclear phagocytes (PMN) to lyse malignant B-cell lines using antibodies and antibody derivates to a range of different B-cell antigens. PMN were found to mediate lysis of all tested Bcell lines in the presence of HLA class II antibodies L227, L243, F3.3, and CR3/43. Target cell lysis was significantly enhanced when PMN isolated during granulocyte ...

A new human leukemia cell line, designated as ME-1, was established from the peripheral blood leukemia cells of a patient with acute myelomonocytic leukemia with eosinophilia (M,E,). This cell line has the characteristic chromosome abnormality of M,E,, inv(l6) (p13q22). When cultured in RPMI 1640 medium containing 10% fetal calf serum, ME-1 cells were monoblastoid, but with the addition of cyto...

The incidence and severity of acute graft-versus-host disease (GVHDJ after allogeneic transplantation using peripheral blood progenitor cells mobilized by granulocyte colonystimulating factor (G-CSF) appear t o be no worse than those after bone marrow transplantation, despite the presence of large numbers of T cells in the donor infusion. Experimental studies have shown that type-l T cells (sec...

Purpose: Anticancer dendritic cell (DC) vaccines require the DCs to relocate to lymph nodes (LN) to trigger immune responses.However, thesemigration rates are typically very poor. Improving the targeting of ex vivo generatedDCs to LNsmight increase vaccine efficacy and reduce costs.We investigatedDCmigration in vivo in humans under different conditions. Experimental Design:HLA-A 02:01 patients ...

Some laboratory results and clinical situations suggest that human T cells may be important in the regulation of growth of hematopoietic cells. Since the discovery of T-cell growth factor (TCGF). systems are now available for the long-term specific in vitro propagation of mature normal or neoplastic human T cells. providing an opportunity to study the influence of T cells on hematopoiesis. Rece...

Use of all-trans-retinoic acid (ATRA) in combinatorial differentiation therapy of acute promyelocytic leukemia (APL) results in exceptional cure rates. However, potent cell differentiation effects of ATRA are so far largely restricted to this disease and long-term survival rates in nonAPL acute myelogeneous leukemia (AML) remain unacceptably poor, requiring development of novel therapeutic stra...

IFN-A is approved for the treatment of multiple cancers. Its pleiotropic properties include inhibition of proliferation and angiogenesis and induction of apoptosis. Type I IFNs also exert immunomodulatory effects, which make it an appropriate candidate to combine with cancer vaccines. The studies reported herein show that 50% of mice reject established B16 tumors following treatment with the co...

Fifty-five hematopoietic cell lines, including 19 T-, 16 B-, S pre-B-, 5 non-T non-B-, 1 erythroid, and 9 myeloid-monocytoidcells, were screened for production of human hematopoietic survival and stem cell growth factor (SCGF) by enzyme immunoassay using anti-SCGF monoclonal antibody. The KPB-M15 myeloid cell line constitutionally secreted a considerable quantity of SCGF, while other Tor myeloi...

Some laboratory results and clinical situations suggest that human T cells may be important in the regulation of growth of hematopoietic cells. Since the discovery of T-cell growth factor (TCGF). systems are now available for the long-term specific in vitro propagation of mature normal or neoplastic human T cells. providing an opportunity to study the influence of T cells on hematopoiesis. Rece...

Nine pediatric patients (median age, 8 years; range, 0.7 to 19 years), eight with refractory aplastic anemia and one with newly diagnosed aplasia, were enrolled in a phase 1/11 trial of recombinant human granulocyte-macrophage colonystimulating factor (rhGM-CSF) administered via continuous intravenous infusion. Doses ranged from 8 to 32 pglkgld. Six of eight evaluable patients responded with a ...