In vitro supplementation with date seed oil (DSO) can protect spermatozoa against hydrogen peroxide (H2O2)-mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the antioxidant effects of DSO on human sperm motility, sperm viability, reacted acrosome and lipid peroxidation assessed in vitro after H2O2-mediated oxidative damage in spermatozoa. Sixtee...

Improved sperm selection techniques are needed to increase the efficiency of equineassisted reproduction. Single layer centrifugation (SLC) of spermatozoa has been shown to improve the quality of stallion sperm samples. In this study, the functionality of selected stallion spermatozoa was tested by intracytoplasmic sperm injection of equine oocytes after selection by SLC through Androcoll-E or ...

In mammals, sperm guidance in the oviduct appears essential for successful sperm arrival at the oocyte. Hitherto, three different potential sperm guidance mechanisms have been recognized: thermotaxis, rheotaxis, and chemotaxis, each of them using specific stimuli - a temperature gradient, fluid flow, and a chemoattractant gradient, respectively. Here, we review sperm behavioral in these mechani...

BACKGROUND Infertility being a burning issue, the male itself contributes about 40% as a cause, as evident by statistical data. However, Assisted Reproductive Technology (ART) has emerged as a powerful tool in the management of infertility. Sperm preparation techniques govern the selection procedure to separate functional spermatozoa which can then be used in IUI, IVF, and ART and for cryoprese...

BACKGROUND Denmark and Norway have a three-fold higher incidence of testicular cancer than Estonia and Finland. Groups of young men from Denmark, Norway, Finland and Estonia were investigated to elucidate whether semen parameters and other related parameters follow a gradient between these countries, as does the gradient in incidence of testicular cancer. METHODS In total, 968 young men from ...

BACKGROUND The intra-cytoplasmic sperm injection (ICSI) technique selects sperm according to morphology and motility. However, these parameters cannot predict the chromatin integrity of sperm. Considering the detrimental effects of DNA-damaged sperm on reproductive outcomes, novel sperm selection procedures have been proposed to circumvent the possibility of inseminating DNA-damaged sperm. It h...

objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

Thermotaxis that sperm migrate to higher temperature area has been confirmed in rabbit and human. In this study, we examined the migration ability of bull sperm in a temperature gradient to confirm thermotaxis and elucidate the involvement of calcium in such thermotaxis, as well as the relation between sperm capacitation and bull fertility. Thermotaxis was evaluated in a temperature gradient of...

Studies showed that assisted reproductive technology changed the sex ratio at birth, but the effect of semen processing (swim-up technique and density-gradient separation) on enriching either Xor Y-bearing sperm is always controversial. We recruited 30 healthy males and their semen samples were obtained by masturbation after sexual abstinence for 2-7 days. Each sample was divided into 3 aliquot...